ArticleThe Journal of membrane biology1995
The Ca(2+)-induced leak current in Xenopus oocytes is indeed mediated through a Cl- channel.
Article in The Journal of membrane biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 40 citations in OpenAlex.
- Two serial filters control P2X7 cation selectivity, Ser342 in the central pore and lateral acidic residues at the cytoplasmic interface.PNAS nexus · 2024Article
- Metabolic energy sensing by mammalian CLC anion/proton exchangers.EMBO reports · 2020Article
- Gating control and KPlant, cell & environment · 2018Article
- Xenopus borealis as an alternative source of oocytes for biophysical and pharmacological studies of neuronal ion channels.Scientific reports · 2015Article
- Calcium rapidly down-regulates human renal epithelial sodium channels via a W-7-sensitive mechanism.The Journal of membrane biology · 2014Article
- Phosphatidylinositol-4,5-bisphosphate (PIP2) regulation of strong inward rectifier Kir2.1 channels: multilevel positive cooperativity.The Journal of physiology · 2008Article
- Kinetic relationship between the voltage sensor and the activation gate in spHCN channels.The Journal of general physiology · 2007Article
- The alphavirus 6K protein activates endogenous ionic conductances when expressed in Xenopus oocytes.The Journal of membrane biology · 2007Article
- Novel outwardly rectifying anion conductance in Xenopus oocytes.Pflugers Archiv : European journal of physiology · 2004Article
- Chloride channel blockers activate an endogenous cationic current in oocytes of Bufo arenarum.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2004Article
- Multiple action sites of flufenamate on ion transport across the rat distal colon.British journal of pharmacology · 2000Article
- Functional and morphological correlates of connexin50 expressed in Xenopus laevis oocytes.The Journal of general physiology · 1999Article
- Regulation of recombinant cardiac cystic fibrosis transmembrane conductance regulator chloride channels by protein kinase C.Biophysical journal · 1999Article
- The ion selectivity of a membrane conductance inactivated by extracellular calcium in Xenopus oocytes.The Journal of physiology · 1998Article
- Extracellular divalent cations block a cation non-selective conductance unrelated to calcium channels in rat cardiac muscle.The Journal of physiology · 1997Article
- Molecular dissection of gating in the ClC-2 chloride channel.The EMBO journal · 1997Article
- cAMP-activation of amiloride-sensitive Na+ channels from guinea-pig colon expressed in Xenopus oocytes.Pflugers Archiv : European journal of physiology · 1996Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Defolliculated oocytes of Xenopus laevis responded to removal of external divalent cations with large depolarizations and, when voltage clamped, with huge currents. Single channel analysis revealed a Cl- channel with a slope conductance of about 90 pS at positive membrane potentials with at least four substates. Single channel amplitudes and mean channel currents had a reversal potential of approximately -15 mV as predicted by the Nernst equation for a channel perfectly selective for Cl-. Readdition of Ca2+ immediately inactivated the channel and restored the former membrane potential or clamp current. The inward currents were mediated by a Ca2+ inactivated Cl- channel (CaIC). The inhibitory potency of Ca2+ was a function of the external Ca2+ concentration with a half maximal blocker concentration of about 20 microM. These channels were inhibited by the Cl- channel blockers flufenamic acid, niflumic acid and diphenylamine-2-carboxylate (DPC). In contrast, 4,4'-acetamido-4'-isothiocyanatostilbene-2, 2'-disulfonicacid (SITS), another Cl- channel blocker, led to activation of this Cl- channel. Like other Cl- channels, the CaIC was activated by cytosolic cAMP. Extracellular ATP inhibited the channel while ADP was without any effect. Injection of phorbol 12-myristate 13-acetate (PMA), a protein kinase C activating phorbol ester, stimulated the Cl- current. Cytochalasin D, an actin filament disrupting compound, reversibly decreased the clamp current demonstrating an influence of the cytoskeleton. The results indicate that removal of divalent cations activates Cl- channels in Xenopus oocytes which share several features with Cl- channels of the CLC family. The former so-called leak current of oocytes under divalent cation-free conditions is nothing else than an activation of Cl- channels.
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