ReviewJournal of bioenergetics and biomembranes1995
Experimental determination of control by the H(+)-ATPase in Escherichia coli.
Review in Journal of bioenergetics and biomembranes, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- How DoesACS synthetic biology · 2024Article
- Escherichia coli robustly expresses ATP synthase at growth rate-maximizing concentrations.The FEBS journal · 2022Article
- Intelligent host engineering for metabolic flux optimisation in biotechnology.The Biochemical journal · 2021Review
- Maintaining maximal metabolic flux by gene expression control.PLoS computational biology · 2018Article
- Evolutionary pressures on microbial metabolic strategies in the chemostat.Scientific reports · 2016Article
- How fast-growing bacteria robustly tune their ribosome concentration to approximate growth-rate maximization.The FEBS journal · 2015Article
- Expression of genes encoding F(1)-ATPase results in uncoupling of glycolysis from biomass production in Lactococcus lactis.Applied and environmental microbiology · 2002Article
- The glycolytic flux in Escherichia coli is controlled by the demand for ATP.Journal of bacteriology · 2002Article
- Stoichiometry of energy coupling by proton-translocating ATPases: a history of variability.Journal of bioenergetics and biomembranes · 2000Review
- atp Mutants of Escherichia coli fail to grow on succinate due to a transport deficiency.Journal of bacteriology · 1998Article
- In situ measurements of creatine kinase flux by NMR. The lessons from bioengineered mice.Molecular and cellular biochemistry · 1998Review
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Strains carrying deletions in the atp genes, encoding the H(+)-ATPase, were unable to grow on nonfermentable substrates such as succinate, whereas with glucose as the substrate the growth rate of an atp deletion mutant was surprisingly high (some 75-80% of wild-type growth rate). The rate of glucose and oxygen consumption of these mutants was increased compared to the wild-type rates. In order to analyze the importance of the H(+)-ATPase at its physiological level, the cellular concentration of H(+)-ATPase was modulated around the wild-type level, using genetically manipulated strains. The control coefficient by the H(+)-ATPase with respect to growth rate and catabolic fluxes was measured. Control on growth rate was absent at the wild-type concentration of H(+)-ATPase, independent of whether the substrate for growth was glucose or succinate. Control by the H(+)-ATPase on the catabolic fluxes, including respiration, was negative at the wild-type H(+)-ATPase level. Moreover, the turnover number of the individual H(+)-ATPase enzymes increased as the H(+)-ATPase concentration was lowered. The negative control by the H(+)-ATPase on catabolism may thus be involved in a homeostatic control of ATP synthesis and, to some extent, explain the zero control by the H(+)-ATPase on E. coli growth rate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.