ArticleThe Journal of physiology1996
Modulation of the hyperpolarization-activated Cl- current in human intestinal T84 epithelial cells by phosphorylation.
Article in The Journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 88 citations in OpenAlex.
- Research and progress on ClC‑2 (Review).Molecular medicine reports · 2017Review
- Development of Automated Patch Clamp Technique to Investigate CFTR Chloride Channel Function.Frontiers in pharmacology · 2017Article
- The principal conductance in Giardia lamblia trophozoites possesses functional properties similar to the mammalian ClC-2 current.Pflugers Archiv : European journal of physiology · 2014Article
- Phenomics of cardiac chloride channels.Comprehensive Physiology · 2013Review
- Lubiprostone activates Cl- secretion via cAMP signaling and increases membrane CFTR in the human colon carcinoma cell line, T84.Digestive diseases and sciences · 2011Article
- Lubiprostone: evaluation of the newest medication for the treatment of adult women with constipation-predominant irritable bowel syndrome.International journal of women's health · 2010Article
- Functional role of CLC-2 chloride inward rectifier channels in cardiac sinoatrial nodal pacemaker cells.Journal of molecular and cellular cardiology · 2009Article
- Lubiprostone stimulates secretion from tracheal submucosal glands of sheep, pigs, and humans.American journal of physiology. Lung cellular and molecular physiology · 2009Article
- Role of intramolecular and intermolecular interactions in ClC channel and transporter function.Pflugers Archiv : European journal of physiology · 2006Review
- Estrogen modulates ClC-2 chloride channel gene expression in rat kidney.Pflugers Archiv : European journal of physiology · 2003Article
- Effect of an N-terminus deletion on voltage-dependent gating of the ClC-2 chloride channel.The Journal of physiology · 2002Article
- Cell cycle- and swelling-induced activation of a Caenorhabditis elegans ClC channel is mediated by CeGLC-7alpha/beta phosphatases.The Journal of cell biology · 2002Article
- Conformation-dependent regulation of inward rectifier chloride channel gating by extracellular protons.The Journal of physiology · 2002Article
- Phosphorylation and functional regulation of ClC-2 chloride channels expressed in Xenopus oocytes by M cyclin-dependent protein kinase.The Journal of physiology · 2002Article
- Inward-rectifying anion channels are expressed in the epithelial cells of choroid plexus isolated from ClC-2 'knock-out' mice.The Journal of physiology · 2002Article
- Regulation of a hyperpolarization-activated chloride current in murine respiratory ciliated cells.The Journal of physiology · 2000Article
- The chloride channel ClC-2 contributes to the inwardly rectifying Cl- conductance in cultured porcine choroid plexus epithelial cells.The Journal of physiology · 2000Article
- Expression of the voltage-gated chloride channel ClC-2 in rod bipolar cells of the rat retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Chloride dependence of hyperpolarization-activated chloride channel gates.The Journal of physiology · 1999Article
- A serine residue in ClC-3 links phosphorylation-dephosphorylation to chloride channel regulation by cell volume.The Journal of general physiology · 1999Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Hyperpolarization-activated Cl- currents (ICl,hyp) were investigated in the T84 human adenocarcinoma cell line, using the patch-clamp whole-cell configuration. 2. During whole-cell recording with high-chloride and ATP-containing internal solutions, hyperpolarizing jumps from a holding potential of 0 mV elicited slow inward current relaxations, carried by Cl- and detected at membrane potentials more negative than -40 mV. Analysis of the relative permeabilities to monovalent anions gave the following sequence: Cl- > Br- > I- > glutamate. 3. ICl,hyp was partially inhibited by 1 mM diphenylamine-2-carboxylic acid or 0.1 mM 5-nitro-2-(3-phenylpropylamino)-benzoate, and was completely blocked by Cd2+ (> 300 microM). It was insensitive to 1 mM external 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid or 1 mM Ba2+. 4. ICl,hyp was inhibited by external application of 500 microM cptcAMP (8-(4-chlorophenylthio)-adenosine 3':5'-cyclic monophosphate) or 500 nM of the protein kinase C activator, phorbol 12-myristate, 13-acetate. 5. (i) Omission of ATP from the pipette solution, (ii) ATP replacement by the non-hydrolysable ATP analogue 5'-adenylylimidodiphosphate, and (iii) inhibition of protein kinase C by staurosporine or calphostin C accelerated the activation kinetics of the current and increased its amplitude, but did not alter its pharmacological properties. 6. We conclude that hyperpolarization-activated Cl- channels similar to those of ClC-2 channels (mammalian homologue of Torpedo chloride channel ClC-0) are present in T84 cells, and that their gating properties are modulated by phosphorylation.
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