ArticleMolecular biology of the cell1996
Expression and activity of serum response factor is required for expression of the muscle-determining factor MyoD in both dividing and differentiating mouse C2C12 myoblasts.
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 77 citations in OpenAlex.
- A novel lncRNA promotes myogenesis of bovine skeletal muscle satellite cells via PFN1-RhoA/Rac1.Journal of cellular and molecular medicine · 2021Article
- Review
- Tyrosine phosphorylation-dependent localization of TmaR that controls activity of a major bacterial sugar regulator by polar sequestration.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Post-transcriptional regulation of MRTF-A by miRNAs during myogenic differentiation of myoblasts.Nucleic acids research · 2020Article
- Basal Signalling Through Death Receptor 5 and Caspase 3 Activates p38 Kinase to Regulate Serum Response Factor (SRF)-Mediated MyoD Transcription.Journal of molecular signaling · 2020Article
- Srf controls satellite cell fusion through the maintenance of actin architecture.The Journal of cell biology · 2018Article
- Reduced nuclear translocation of serum response factor is associated with skeletal muscle atrophy in a cigarette smoke-induced mouse model of COPD.International journal of chronic obstructive pulmonary disease · 2017Article
- Serum Response Factor in Muscle Tissues: From Development to Ageing.European journal of translational myology · 2016Article
- Current understanding of sarcopenia: possible candidates modulating muscle mass.Pflugers Archiv : European journal of physiology · 2015Review
- The STARS signaling pathway: a key regulator of skeletal muscle function.Pflugers Archiv : European journal of physiology · 2014Review
- Age-related decreases of serum-response factor levels in human mesenchymal stem cells are involved in skeletal muscle differentiation and engraftment capacity.Stem cells and development · 2014Article
- The intriguing regulators of muscle mass in sarcopenia and muscular dystrophy.Frontiers in aging neuroscience · 2014Review
- G-protein coupled receptor 56 promotes myoblast fusion through serum response factor- and nuclear factor of activated T-cell-mediated signalling but is not essential for muscle development in vivo.The FEBS journal · 2013Article
- MicroRNA-199a is induced in dystrophic muscle and affects WNT signaling, cell proliferation, and myogenic differentiation.Cell death and differentiation · 2013Article
- miR-411 is up-regulated in FSHD myoblasts and suppresses myogenic factors.Orphanet journal of rare diseases · 2013Article
- Myod and H19-Igf2 locus interactions are required for diaphragm formation in the mouse.Development (Cambridge, England) · 2013Article
- Satellite cells and the muscle stem cell niche.Physiological reviews · 2013Review
- MASTR directs MyoD-dependent satellite cell differentiation during skeletal muscle regeneration.Genes & development · 2012Article
- Dystrophin Orchestrates the Epigenetic Profile of Muscle Cells Via miRNAs.Frontiers in genetics · 2011Article
- Regulation of Serum Response Factor and Adiponectin by PPARγ Agonist Docosahexaenoic Acid.Journal of lipids · 2011Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand the mechanism by which the serum response factor (SRF) is involved in the process of skeletal muscle differentiation, we have assessed the effect of inhibiting SRF activity or synthesis on the expression of the muscle-determining factor MyoD. Inhibition of SRF activity in mouse myogenic C2C12 cells through microinjection of either the SRE oligonucleotide (which acts by displacing SRF proteins from the endogenous SRE sequences), purified SRF-DB (a 30-kDa portion of SRF containing the DNA-binding domain of SRF, which acts as a dominant negative mutant in vivo), or purified anti-SRF antibodies rapidly prevents the expression of MyoD. Moreover, the rapid shutdown of MyoD expression after in vivo inhibition of SRF activity is observed not only in proliferating myoblasts but also in myoblasts cultured under differentiating conditions. Additionally, by using a cellular system expressing a glucocorticoid-inducible antisense-SRF (from aa 74 to 244) we have shown that blocking SRF expression by dexamethasone induction of antisense SRF results in the lack of MyoD expression as probed by both immunofluorescence and Northern blot analysis. Taken together these data demonstrate that SRF expression and activity are required for the expression of the muscle-determining factor MyoD.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.