ArticleMolecular biology of the cell1996
Distinct roles of yeast MEC and RAD checkpoint genes in transcriptional induction after DNA damage and implications for function.
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 77 citations in OpenAlex.
- Separable roles of the DNA damage response kinase Mec1ATR and its activator Rad24RAD17 during meiotic recombination.PLoS genetics · 2024Article
- Protein phosphatase 2A (PP2A) promotes anaphase entry after DNA replication stress in budding yeast.Molecular biology of the cell · 2021Article
- Review
- Checkpoints couple transcription network oscillator dynamics to cell-cycle progression.Genome biology · 2014Article
- The subunits of the S-phase checkpoint complex Mrc1/Tof1/Csm3: dynamics and interdependence.Cell division · 2014Article
- The checkpoint transcriptional response: make sure to turn it off once you are satisfied.Cell cycle (Georgetown, Tex.) · 2012Article
- Functional genomics of genes with small open reading frames (sORFs) in S. cerevisiae.Genome research · 2006Article
- The budding yeast protein Chl1p is required to preserve genome integrity upon DNA damage in S-phase.Nucleic acids research · 2006Article
- Loss of SOD1 and LYS7 sensitizes Saccharomyces cerevisiae to hydroxyurea and DNA damage agents and downregulates MEC1 pathway effectors.Molecular and cellular biology · 2005Article
- A haploid-specific transcriptional response to irradiation in Saccharomyces cerevisiae.Nucleic acids research · 2005Article
- A role for Saccharomyces cerevisiae Chk1p in the response to replication blocks.Molecular biology of the cell · 2004Article
- Pdr3 is required for DNA damage induction of MAG1 and DDI1 via a bi-directional promoter element.Nucleic acids research · 2004Article
- The checkpoint protein Rad24 of Saccharomyces cerevisiae is involved in processing double-strand break ends and in recombination partner choice.Molecular and cellular biology · 2003Article
- A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Parallel identification of new genes in Saccharomyces cerevisiae.Genome research · 2002Article
- Phosphorylation of Rph1, a damage-responsive repressor of PHR1 in Saccharomyces cerevisiae, is dependent upon Rad53 kinase.Nucleic acids research · 2002Article
- Topoisomerase III acts upstream of Rad53p in the S-phase DNA damage checkpoint.Molecular and cellular biology · 2001Article
- Article
- The proteasome regulates the UV-induced activation of the AP-1-like transcription factor Gcn4.Genes & development · 2001Article
- Rdp1, a novel zinc finger protein, regulates the DNA damage response of rhp51(+) from Schizosaccharomyces pombe.Molecular and cellular biology · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In eukaryotic cells, checkpoint genes cause arrest of cell division when DNA is damaged or when DNA replication is blocked. In this study of budding yeast checkpoint genes, we identify and characterize another role for these checkpoint genes after DNA damage-transcriptional induction of genes. We found that three checkpoint genes (of six genes tested) have strong and distinct roles in transcriptional induction in four distinct pathways of regulation (each defined by induction of specific genes). MEC1 mediates the response in three transcriptional pathways, RAD53 mediates two of these pathways, and RAD17 mediates but a single pathway. The three other checkpoint genes (including RAD9) have small (twofold) but significant roles in transcriptional induction in all pathways. One of the pathways that we identify here leads to induction of MEC1 and RAD53 checkpoint genes themselves. This suggests a positive feedback circuit that may increase the cell's ability to respond to DNA damage. We make two primary conclusions from these studies. First, MEC1 appears to be the key regulator because it is required for all responses (both transcriptional and cell cycle arrest), while other genes serve only a subset of these responses. Second, the two types of responses, transcriptional induction and cell cycle arrest, appear distinct because both require MEC1 yet only cell cycle arrest requires RAD9. These and other results were used to formulate a working model of checkpoint gene function that accounts for roles of different checkpoint genes in different responses and after different types of damage. The conclusion that the yeast MEC1 gene is a key regulator also has implications for the role of a putative human homologue, the ATM gene.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.