ArticleBiophysical journal1996
Subunit interactions in the activation of cyclic nucleotide-gated ion channels.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 75 citations in OpenAlex.
- The allosteric gating mechanism of the MthK channel.National science review · 2022Article
- Article
- Structure and Energetics of Allosteric Regulation of HCN2 Ion Channels by Cyclic Nucleotides.The Journal of biological chemistry · 2016Article
- Use of concatemers of ligand-gated ion channel subunits to study mechanisms of steroid potentiation.Anesthesiology · 2011Review
- Ligand-binding domain subregions contributing to bimodal agonism in cyclic nucleotide-gated channels.The Journal of general physiology · 2011Article
- Mutations reveal voltage gating of CNGA1 channels in saturating cGMP.The Journal of general physiology · 2009Article
- A comparison of electrophysiological properties of the CNGA1, CNGA1tandem and CNGA1cys-free channels.European biophysics journal : EBJ · 2008Article
- Distribution, amplification, and summation of cyclic nucleotide sensitivities within single olfactory sensory cilia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- The pharmacology of cyclic nucleotide-gated channels: emerging from the darkness.Current pharmaceutical design · 2006Review
- Activation of olfactory-type cyclic nucleotide-gated channels is highly cooperative.The Journal of physiology · 2005Article
- Calcium activation of ryanodine receptor channels--reconciling RyR gating models with tetrameric channel structure.The Journal of general physiology · 2005Article
- Modifications to the tetracaine scaffold produce cyclic nucleotide-gated channel blockers with widely varying efficacies.Journal of medicinal chemistry · 2005Article
- Tyrosine phosphorylation of rod cyclic nucleotide-gated channels switches off Ca2+/calmodulin inhibition.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- A cysteine scan of the inner vestibule of cyclic nucleotide-gated channels reveals architecture and rearrangement of the pore.The Journal of general physiology · 2003Article
- Stepwise contribution of each subunit to the cooperative activation of BK channels by Ca2+.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Mechanism of calcium/calmodulin inhibition of rod cyclic nucleotide-gated channels.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Molecular regions controlling the activity of CNG channels.The Journal of general physiology · 2001Article
- The interaction of Na(+) and K(+) in the pore of cyclic nucleotide-gated channels.Biophysical journal · 2000Article
- Effects of ultraviolet modification on the gating energetics of cyclic nucleotide-gated channels.The Journal of general physiology · 2000Article
- Structural basis for ligand selectivity of heteromeric olfactory cyclic nucleotide-gated channels.Biophysical journal · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Cyclic nucleotide-gated (CNG) ion channels of retinal photoreceptors and olfactory neurons are multimeric proteins of unknown stoichiometry. To investigate the subunit interactions that occur during CNG channel activation, we have used tandem cDNA constructs of the rod CNG channel to generate heteromultimeric channels composed of wild-type and mutant subunits. We introduced point mutations that affect channel activation: 1) D604M, which alters the relative ability of agonists to promote the allosteric conformational change(s) associated with channel opening, and 2) T560A, which primarily affects the initial binding affinity for cGMP, and to a lesser extent, the allosteric transition. At saturating concentrations of agonist, heteromultimeric channels were intermediate between wild-type and mutant homomultimers in agonist efficacy and apparent affinity for cGMP, cIMP, and cAMP, consistent with a model for the allosteric transition involving a concerted conformational change in all of the channel subunits. Results were also consistent with a model involving independent transitions in two or three, but not one or four, of the channel subunits. The behavior of the heterodimers implies that the channel stoichiometry is some multiple of 2 and is consistent with a tetrameric quaternary structure for the functional channel complex. Steady-state dose-response relations for homomultimeric and heteromultimeric channels were well fit by a Monod, Wyman, and Changeux model with a concerted allosteric opening transition stabilized by binding of agonist.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.