ArticleThe Plant cell1996
A soybean sucrose binding protein independently mediates nonsaturable sucrose uptake in yeast.
Article in The Plant cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 62 citations in OpenAlex.
- Article
- Trimethylguanosine Synthase1 (TGS1) Is Essential for Chilling Tolerance.Plant physiology · 2017Article
- Type 1 diacylglycerol acyltransferases of Brassica napus preferentially incorporate oleic acid into triacylglycerol.Journal of experimental botany · 2015Article
- A 64 kDa sucrose binding protein is membrane-associated and tonoplast-localized in developing mung bean seeds.Journal of experimental botany · 2009Article
- Intracellular sucrose communicates metabolic demand to sucrose transporters in developing pea cotyledons.Journal of experimental botany · 2009Article
- Distinct repressing modules on the distal region of the SBP2 promoter contribute to its vascular tissue-specific expression in different vegetative organs.Plant molecular biology · 2007Article
- Promoter activity of a putative pollen monosaccharide transporter in Petunia hybrida and characterisation of a transposon insertion mutant.Protoplasma · 2006Article
- Sucrose transport into plasma membrane vesicles from tobacco leaves by H+ symport or counter exchange does not display a linear component.The Journal of membrane biology · 2004Article
- Protein sorting and expression of a unique soybean cotyledon protein, GmSBP, destined for the protein storage vacuole.Plant molecular biology · 2003Article
- Expression patterns and subcellular localization of a 52 kDa sucrose-binding protein homologue of Vicia faba (VfSBPL) suggest different functions during development.Plant molecular biology · 2001Article
- A new subfamily of sucrose transporters, SUT4, with low affinity/high capacity localized in enucleate sieve elements of plants.The Plant cell · 2000Article
- Rates of sugar uptake by guard cell protoplasts of pisum sativum L. Related To the solute requirement for stomatal openingPlant physiology · 1999Article
- The dual function of sugar carriers. Transport and sugar sensingThe Plant cell · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterologous expression of a cDNA encoding a 62-kD soybean sucrose binding protein in yeast demonstrates that this protein, independent of other plant proteins, mediates sucrose uptake across the plasma membrane. Sucrose binding protein-mediated sucrose uptake is nonsaturable up to 30 mM sucrose, is specific for sucrose, and is relatively insensitive to treatment with sulfhydryl-modifying reagents. Alteration of the external pH or pretreatment of the yeast cells with protonophores did not significantly affect the rate of 14C-sucrose uptake. This demonstrates that sucrose binding protein-mediated sucrose uptake is not dependent on H+ movement and delineates it from other plant sucrose transporters. Physiological characterization of sucrose uptake into higher plant cells has shown the presence of both saturable and nonsaturable uptake components. The nonsaturable mechanism is relatively insensitive to external pH, pretreatment with protonophores, and treatment with sulfhydryl-modifying reagents. Sucrose binding protein-mediated sucrose uptake in yeast mimics this physiologically described, but mechanistically undefined, nonsaturable sucrose uptake mechanism in higher plants. Functional characterization of the sucrose binding protein thus defines both a novel component of sucrose uptake and provides important insight into this nonsaturable sucrose uptake mechanism, which has remained enigmatic since its physiological description.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.