ArticlePlant physiology1996
Molecular cloning, immunochemical localization to the vacuole, and expression in transgenic yeast and tobacco of a putative sugar transporter from sugar beet.
Article in Plant physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 71 citations in OpenAlex.
- Modifications in Carbon and Nitrogen Metabolites ofPlants (Basel, Switzerland) · 2025Article
- The Identification, Characterization, and Functional Analysis of the Sugar Transporter Gene Family of the Rice False Smut Pathogen,International journal of molecular sciences · 2024Article
- Outlier analyses and genome-wide association study identifyFrontiers in plant science · 2022Article
- The functional analysis of sugar transporter proteins in sugar accumulation and pollen tube growth in pummelo (Frontiers in plant science · 2022Article
- Identifying the pathways that control resource allocation in higher plants.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Soybean LEC2 Regulates Subsets of Genes Involved in Controlling the Biosynthesis and Catabolism of Seed Storage Substances and Seed Development.Frontiers in plant science · 2017Article
- Role of metabolite transporters in source-sink carbon allocation.Frontiers in plant science · 2013Article
- A novel Arabidopsis vacuolar glucose exporter is involved in cellular sugar homeostasis and affects the composition of seed storage compounds.Plant physiology · 2011Article
- Functional analysis of an Arabidopsis thaliana abiotic stress-inducible facilitated diffusion transporter for monosaccharides.The Journal of biological chemistry · 2010Article
- Transcriptome analysis of a spontaneous mutant in sweet orange [Citrus sinensis (L.) Osbeck] during fruit development.Journal of experimental botany · 2009Article
- Functional and physiological characterization of Arabidopsis INOSITOL TRANSPORTER1, a novel tonoplast-localized transporter for myo-inositol.The Plant cell · 2008Article
- Molecular cloning and expression analysis of a monosaccharide transporter gene OsMST4 from rice (Oryza sativa L.).Plant molecular biology · 2007Article
- A proteomics dissection of Arabidopsis thaliana vacuoles isolated from cell culture.Molecular & cellular proteomics : MCP · 2007Article
- Identification and functional expression of the Arabidopsis thaliana vacuolar glucose transporter 1 and its role in seed germination and flowering.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Molecular identification and physiological characterization of a novel monosaccharide transporter from Arabidopsis involved in vacuolar sugar transport.The Plant cell · 2006Article
- Identification of a vacuolar sucrose transporter in barley and Arabidopsis mesophyll cells by a tonoplast proteomic approach.Plant physiology · 2006Article
- Expression of a tomato sugar transporter is increased in leaves of mycorrhizal or Phytophthora parasitica-infected plants.Mycorrhiza · 2005Article
- Structure-function analysis of the presumptive Arabidopsis auxin permease AUX1.The Plant cell · 2004Article
- Cloning, expression, and characterization of sorbitol transporters from developing sour cherry fruit and leaf sink tissues.Plant physiology · 2003Article
- Analysis of the compartmentation of glycolytic intermediates, nucleotides, sugars, organic acids, amino acids, and sugar alcohols in potato tubers using a nonaqueous fractionation method.Plant physiology · 2001Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several plant genes have been cloned that encode members of the sugar transporter subgroup of the major facilitator superfamily of transporters. Here we report the cloning, expression, and membrane localization of one of these porters found in sugar beet (Beta vulgaris L.). This clone, cDNA-1, codes for a protein with 490 amino acids and an estimated molecular mass of 54 kD. The predicted membrane topology and sequence homology suggest that cDNA-1 is a member of the sugar transporter family. RNA gel blot analysis revealed that this putative sugar transporter is expressed in all vegetative tissues and expression increases with development in leaves. DNA gel blot analysis indicated that multiple gene copies exist for this putative sugar transporter in the sugar beet genome. Antibodies directed against small peptides representing the N- and C-terminal domains of the cDNA1 protein identified a 40-kD polypeptide in microsomes isolated from cDNA-1-transformed yeast (Saccharomyces cerevisiae). Moreover, the same protein was identified in sugar beet and transgenic tobacco (Nicotaina tobacum L.) membrane fractions. Detailed analysis of the transporter's distribution across linear sucrose gradients and flotation centrifugations showed that it co-migrates with tonoplast membrane markers. We conclude that this carrier is located on the tonoplast membrane and that it may mediate sugar partitioning between the vacuole and cytoplasmic compartments.
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