ArticleMolecular biology of the cell1996
Mutational analysis of capping protein function in Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- F-Actin Cytoskeleton Network Self-Organization Through Competition and Cooperation.Annual review of cell and developmental biology · 2020Review
- Physiological role of actin regulation in male fertility: Insight into actin capping proteins in spermatogenic cells.Reproductive medicine and biology · 2020Article
- Capping Protein Insulates Arp2/3-Assembled Actin Patches from Formins.Current biology : CB · 2019Article
- Allosteric Coupling of CARMIL and V-1 Binding to Capping Protein Revealed by Hydrogen-Deuterium Exchange.Cell reports · 2018Article
- Internetwork competition for monomers governs actin cytoskeleton organization.Nature reviews. Molecular cell biology · 2016Article
- Heterodimeric capping protein from Arabidopsis is a membrane-associated, actin-binding protein.Plant physiology · 2014Article
- Capping protein regulators fine-tune actin assembly dynamics.Nature reviews. Molecular cell biology · 2014Review
- Capping protein modulates the dynamic behavior of actin filaments in response to phosphatidic acid in Arabidopsis.The Plant cell · 2012Article
- Requirements of Slm proteins for proper eisosome organization, endocytic trafficking and recycling in the yeast Saccharomyces cerevisiae.Journal of biosciences · 2011Article
- Molecular basis for barbed end uncapping by CARMIL homology domain 3 of mouse CARMIL-1.The Journal of biological chemistry · 2010Article
- Structural basis for capping protein sequestration by myotrophin (V-1).The Journal of biological chemistry · 2010Article
- Direct observation of the uncapping of capping protein-capped actin filaments by CARMIL homology domain 3.The Journal of biological chemistry · 2010Article
- New insights into mechanism and regulation of actin capping protein.International review of cell and molecular biology · 2008Review
- Structural basis of actin filament capping at the barbed-end: a cryo-electron microscopy study.The EMBO journal · 2006Article
- Heterodimeric capping protein from Arabidopsis is regulated by phosphatidic acid.Molecular biology of the cell · 2006Article
- Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance.The Journal of cell biology · 2004Article
- Crystal structure of CapZ: structural basis for actin filament barbed end capping.The EMBO journal · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
To investigate physiologic functions and structural correlates for actin capping protein (CP), we analyzed site-directed mutations in CAP1 and CAP2, which encode the alpha and beta subunits of CP in Saccharomyces cerevisiae. Mutations in four different regions caused a loss of CP function in vivo despite the presence of mutant protein in the cells. Mutations in three regions caused a complete loss of all aspects of function, including the actin distribution, viability with sac6, and localization of CP to actin cortical patches. Mutation of the fourth region led to partial loss of only one function-formation of actin cables. Some mutations retained function and exhibited the complete wild-type phenotype, and some mutations led to a complete loss of protein and therefore loss of function. The simplest hypothesis that can explain these results is that a single biochemical property is necessary for all in vivo functions. This biochemical property is most likely binding to actin filaments, because the nonfunctional mutant CPs no longer co-localize with actin filaments in vivo and because direct binding of CP to actin filaments has been well established by studies with purified proteins in vitro. More complex hypotheses, involving the existence of additional biochemical properties important for function, cannot be excluded by this analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.