ArticleJournal of clinical microbiology1996
Genomic fingerprinting for epidemiological differentiation of Staphylococcus aureus clinical isolates.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Murine immune response to a chronic Staphylococcus aureus biofilm infection.Infection and immunity · 2011Article
- The antifungal vaccine derived from the recombinant N terminus of Als3p protects mice against the bacterium Staphylococcus aureus.Infection and immunity · 2008Article
- Comparative genomics of Staphylococcus aureus musculoskeletal isolates.Journal of bacteriology · 2005Article
- Molecular diagnostics of clinically important staphylococci.Folia microbiologica · 2004Review
- Strain-dependent differences in the regulatory roles of sarA and agr in Staphylococcus aureus.Infection and immunity · 2002Article
- Multiplex PCR protocol for the diagnosis of staphylococcal infection.Journal of clinical microbiology · 2001Article
- Validation of binary typing for Staphylococcus aureus strains.Journal of clinical microbiology · 1999Article
- Factors affecting the collagen binding capacity of Staphylococcus aureus.Infection and immunity · 1998Article
- Comparative evaluation of use of cna, fnbA, fnbB, and hlb for genomic fingerprinting in the epidemiological typing of Staphylococcus aureus.Journal of clinical microbiology · 1997Article
- Contribution of a typing method based on IS256 probing of SmaI-digested cellular DNA to discrimination of European phage type 77 methicillin-resistant Staphylococcus aureus strains.Journal of clinical microbiology · 1997Article
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Authors and funding
4 authors.
Funding
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Abstract
We used genomic fingerprinting to investigate an outbreak of methicillin-resistant Staphylococcus aureus in the neonatal intensive care units (NICUs) of two hospitals. The hospitals are located in the same city and are part of the same medical care system. Fingerprinting was done by Southern blot hybridization with DNA probes for the genes encoding the S. aureus collagen adhesin (cna), fibronectin-binding proteins (fnbA and fnbB), and beta-toxin (hlb). Genomic DNA was digested with HaeIII (cna and fnbA-fnbB probes) or HindIII (hlb probe). Hybridization patterns could be distinguished on the basis of (i) the presence or absence of cna, (ii) the size of the restriction fragment containing the cna gene, (iii) restriction fragment length polymorphisms within fnbA and fnbB, (iv) the presence of a lysogenic phage within hlb, and (v) the sizes of the restriction fragments containing the phage-bacterial DNA junction fragments. Over a period of 4 months we examined a total of 46 isolates obtained from various wards within each hospital. Among these 46 isolates, we observed a total of 4 cna patterns, 11 fnbA-fnbB patterns, and 11 hlb patterns. Southern blots with HaeIII-digested genomic DNA and a combination of all three gene probes revealed a total of 16 clearly distinguishable patterns. A total of 22 of the 46 isolates were identical with respect to every genomic marker examined. A total of 21 of these 22 isolates were obtained from patients within an NICU. Nineteen of 21 isolates also exhibited identical antibiotic resistance profiles (antibiogram). Although 5 of the remaining 24 strains exhibited an antibiogram identical to those of the NICU isolates, all 24 strains could be distinguished from the NICU isolates by at least one genomic marker. These results suggest that the NICU isolates had a common origin and that genomic fingerprinting with the cna, fnbA, fnbB, and hlb gene probes can provide an important epidemiological tool for the identification of clinical isolates of S. aureus.
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