ArticleProtein science : a publication of the Protein Society1996
Probing the tertiary structure of proteins by limited proteolysis and mass spectrometry: the case of Minibody.
Article in Protein science : a publication of the Protein Society, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Thymic Stromal Lymphopoietin (TSLP) Is Cleaved by Human Mast Cell Tryptase and Chymase.International journal of molecular sciences · 2024Article
- Article
- Protein Structural Analysis via Mass Spectrometry-Based Proteomics.Advances in experimental medicine and biology · 2016Review
- Limited proteolysis of fibrinogen by fibrinogenase from Echis multisquamatis venom.The protein journal · 2015Article
- A new structural insight into XPA-DNA interactions.Bioscience reports · 2014Article
- Structure-specific ribonucleases for MS-based elucidation of higher-order RNA structure.Journal of the American Society for Mass Spectrometry · 2014Article
- Protein design: toward functional metalloenzymes.Chemical reviews · 2014Review
- Structure-function analysis of the short splicing variant carboxypeptidase encoded by Drosophila melanogaster silver.Journal of molecular biology · 2010Article
- On the relevance of the Met-turn methionine in metzincins.The Journal of biological chemistry · 2010Article
- Nanotools for megaproblems: probing protein misfolding diseases using nanomedicine modus operandi.Journal of proteome research · 2006Review
- Modulation of replication protein A function by its hyperphosphorylation-induced conformational change involving DNA binding domain B.The Journal of biological chemistry · 2005Article
- Deciphering structure and topology of conserved COG2042 orphan proteins.BMC structural biology · 2005Article
- Structural characterization of Escherichia coli sensor histidine kinase EnvZ: the periplasmic C-terminal core domain is critical for homodimerization.The Biochemical journal · 2005Article
- Ribosomal protein L7a binds RNA through two distinct RNA-binding domains.The Biochemical journal · 2005Article
- Binding and relaxometric properties of heme complexes with cyanogen bromide fragments of human serum albumin.Biophysical journal · 2002Article
- Topological investigation of amyloid fibrils obtained from beta2-microglobulin.Protein science : a publication of the Protein Society · 2002Article
- The effect of prime-site occupancy on the hepatitis C virus NS3 protease structure.Protein science : a publication of the Protein Society · 2002Article
- Description of the topographical changes associated to the different stages of the DsbA catalytic cycle.Protein science : a publication of the Protein Society · 2002Article
- Conformational analysis of putative regulatory subunit D of the toluene/o-xylene-monooxygenase complex from Pseudomonas stutzeri OX1.Protein science : a publication of the Protein Society · 2001Article
- Removal of the N-terminal hexapeptide from human beta2-microglobulin facilitates protein aggregation and fibril formation.Protein science : a publication of the Protein Society · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A strategy that combines limited proteolysis experiments and mass spectrometric analysis of the fragments generated has been developed to probe protease-accessible sites on the protein surface. This integrated approach has been employed to investigate the tertiary structure of the Minibody, a de novo designed 64-residue protein consisting of a beta-sheet scaffold based on the heavy-chain variable-domain structure of a mouse immunoglobulin and containing two segments corresponding to the hypervariable H1 and H2 regions. The low solubility of the protein prevented a detailed characterization by NMR and/or X-ray. Different proteases were used under strictly controlled conditions and the cleavage sites were mapped onto the anticipated Minibody model, leading to the identification of the most exposed regions. A single-residue mutant was constructed and characterized, following the same procedure, showing a slightly higher correspondence with the predicted model. This strategy can be used to effectively supplement NMR and X-ray investigations of protein tertiary structure, where these procedures cannot provide definitive data, or to verify and refine protein models.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.