ArticleThe Journal of physiology1996
Depolarization-evoked increases in cytosolic calcium concentration in isolated smooth muscle cells of rat portal vein.
Article in The Journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 78 citations in OpenAlex.
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- Physiology of intracellular calcium buffering.Physiological reviews · 2023Review
- Aldosterone-Induced Sarco/Endoplasmic Reticulum CaFrontiers in physiology · 2022Article
- Voltage-gated CaAmerican journal of physiology. Heart and circulatory physiology · 2018Article
- Regulation of Coronary Blood Flow.Comprehensive Physiology · 2017Review
- Examining the role of mitochondria in Ca²⁺ signaling in native vascular smooth muscle.Microcirculation (New York, N.Y. : 1994) · 2013Review
- Mitochondrial regulation of cytosolic Ca²⁺ signals in smooth muscle.Pflugers Archiv : European journal of physiology · 2012Review
- Article
- Ca2+ entry following P2X receptor activation induces IP3 receptor-mediated Ca2+ release in myocytes from small renal arteries.British journal of pharmacology · 2011Article
- Indirect coupling between Cav1.2 channels and ryanodine receptors to generate Ca2+ sparks in murine arterial smooth muscle cells.The Journal of physiology · 2007Article
- Ryanodine receptor type 2 deficiency changes excitation-contraction coupling and membrane potential in urinary bladder smooth muscle.The Journal of physiology · 2007Article
- Two distinct signaling pathways for regulation of spontaneous local Ca2+ release by phospholipase C in airway smooth muscle cells.Pflugers Archiv : European journal of physiology · 2007Article
- IP3-mediated Ca2+ increases do not involve the ryanodine receptor, but ryanodine receptor antagonists reduce IP3-mediated Ca2+ increases in guinea-pig colonic smooth muscle cells.The Journal of physiology · 2005Article
- Type-3 ryanodine receptors mediate hypoxia-, but not neurotransmitter-induced calcium release and contraction in pulmonary artery smooth muscle cells.The Journal of general physiology · 2005Article
- Effects of L-arginine on spontaneous contraction of the rat portal vein.Pflugers Archiv : European journal of physiology · 2003Article
- Ca(2+) regulation in guinea-pig colonic smooth muscle: the role of the Na(+)-Ca(2+) exchanger and the sarcoplasmic reticulum.The Journal of physiology · 2002Article
- Two Ca2+ entry pathways mediate InsP3-sensitive store refilling in guinea-pig colonic smooth muscle.The Journal of physiology · 2000Article
- Calcium-induced calcium release in smooth muscle: loose coupling between the action potential and calcium release.The Journal of general physiology · 2000Article
- Multiple pathways responsible for the stretch-induced increase in Ca2+ concentration in toad stomach smooth muscle cells.The Journal of physiology · 2000Article
- Mechanisms that regulate [Ca2+]i following depolarization in rat systemic arterial smooth muscle cells.The Journal of physiology · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
1. Ca2+ current through voltage-dependent Ca2+ channels (ICa) and intracellular free Ca2+ concentration ([Ca2+]i) were measured simultaneously in rat portal vein smooth muscle cells using conventional whole-cell voltage clamp technique and high temporal resolution microfluorimetry. 2. The relationship between depolarization-evoked ICa and rise in [Ca2+]i was examined. The extracellular Ca2+ concentration dependence and the voltage dependence of the depolarization-evoked increases in ICa and [Ca2+]i were similar. Both ICa and increased [Ca2+]i were blocked to a similar extent by nimodipine and cadmium and augmented by Bay K 8644. Furthermore, the time course of the measured increase in [Ca2+]i, closely followed the increase in [Ca2+]i expected from the time-integrated ICa. These observations suggest that the depolarization-evoked rise in [Ca2+]i was tightly coupled to ICa. 3. The cytosolic Ca2+ buffering capacity, determined as the ratio of the expected increase in [Ca2+]i (from ICa) divided by the measured increase in [Ca2+]i, was over 100. Therefore, less than 1 out of 100 Ca2+ ions entering the cell appears as a free Ca2+. 4. Ryanodine (30 microM), a blocker of the Ca(2+)-induced Ca2+ release mechanism, had little effect on buffering capacity measured over the first 200 ms of the depolarizing voltage clamp pulse. Ryanodine also had little effect on the buffering capacity during 800-1000 ms of the depolarizing voltage clamp pulse. Therefore, it was concluded that there is little Ca(2+)-induced Ca2+ release from the stores in rat portal vein smooth muscle cells during depolarization-evoked Ca2+ entry. 5. During brief depolarizations, the largest [Ca2+]i increase and ICa occurred at 0 mV. However, during steady-state depolarization, the largest increase in [Ca2+]i occurred around -30 mV, and we estimate the peak steady-state ICa to be about 0.6 pA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.