ArticleJournal of clinical microbiology1996
Evaluation of mtp40 genomic fragment amplification for specific detection of Mycobacterium tuberculosis in clinical specimens.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Performance of nested multiplex PCR assay targeting MTP40 and IS6110 gene sequences for the diagnosis of tubercular lymphadenitis.Journal of microbiology (Seoul, Korea) · 2017Article
- Differentiation of Mycobacterium tuberculosis complex from non-tubercular mycobacteria by nested multiplex PCR targeting IS6110, MTP40 and 32kD alpha antigen encoding gene fragments.BMC infectious diseases · 2016Article
- Clinical evaluation of mtp40 polymerase chain reaction for the diagnosis of extra pulmonary tuberculosis.World journal of microbiology & biotechnology · 2014Article
- Protein-coding housekeeping gene Rv2461c can be used as an amplification target in loop-mediated isothermal amplification assay for the detection of Mycobacterium tuberculosis in sputum samples.International journal of clinical and experimental pathology · 2014Article
- Article
- Molecular and histopathologic evidence for systemic infection by Mycobacterium bovis in a patient with tuberculous enteritis, peritonitis, and meningitis: a case report.The Kaohsiung journal of medical sciences · 2004Article
- Specificities and functions of the recA and pps1 intein genes of Mycobacterium tuberculosis and application for diagnosis of tuberculosis.Journal of clinical microbiology · 2002Article
- A PCR-colorimetric microwell plate hybridization assay for detection of Mycobacterium tuberculosis and M. avium from culture samples and Ziehl-Neelsen-positive smears.Journal of clinical microbiology · 2000Article
- Evaluation of PCR in detection of Mycobacterium tuberculosis from formalin-fixed, paraffin-embedded tissues: comparison of four amplification assays.Journal of clinical microbiology · 1998Article
- Identification of a new DNA region specific for members of Mycobacterium tuberculosis complex.Journal of clinical microbiology · 1998Article
- Absence of the genetic marker IS6110 from a strain of Mycobacterium tuberculosis isolated in Ontario.The Canadian journal of infectious diseases = Journal canadien des maladies infectieuses · 1998Article
- Relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory.Clinical microbiology reviews · 1997Review
- Characterization of mycobacterial isolates phylogenetically related to, but different from Mycobacterium simiae.Journal of clinical microbiology · 1997Article
- Specific detection of Mycobacterium tuberculosis by mtp40 nested PCR.Journal of clinical microbiology · 1996Article
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Abstract
A PCR assay based on the species-specific mtp40 genomic fragment was developed for the specific detection and identification of Mycobacterium tuberculosis in different uncultured clinical specimens. The aim of the study was to evaluate the clinical applicability of this target DNA in comparison with those of conventional microbiological methods and to compare the results obtained with those obtained after amplification with the IS6110 repetitive element. Discrepant results were interpreted in conjunction with the patients' clinical data, medical histories, and response to therapy. A total of 172 specimens from 162 patients with respiratory symptoms were tested, 101 specimens were obtained from 92 patients clinically suspected of having tuberculosis, and 71 specimens were obtained from 70 patients without known mycobacterial infection. The results of our study suggest that PCR amplification with the mtp40 genomic fragment provides a highly sensitive and specific technique for the detection of M. tuberculosis strains in clinical samples. It allows for the differentiation between M. tuberculosis and other related mycobacteria, including M. bovis, and is more specific than the IS6110 target. For these and other reasons, we propose that the mtp40 assay is a possible alternative for the specific direct detection of M. tuberculosis in clinical laboratories.
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