ArticleGenetics1996
Cold-sensitive mutations of Dictyostelium myosin heavy chain highlight functional domains of the myosin motor.
Article in Genetics, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 36 citations in OpenAlex.
- Electrostatic interactions in the force-generating region of the human cardiac myosin modulate ADP dissociation from actomyosin.Biochemical and biophysical research communications · 2019Article
- Mutations in the SH1 helix alter the thermal properties of myosin II.Biophysics and physicobiology · 2017Article
- Rational elicitation of cold-sensitive phenotypes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Article
- Molecular motors: forty years of interdisciplinary research.Molecular biology of the cell · 2011Article
- Structural basis for the allosteric interference of myosin function by reactive thiol region mutations G680A and G680V.The Journal of biological chemistry · 2011Article
- Allosteric transitions in biological nanomachines are described by robust normal modes of elastic networks.Current protein & peptide science · 2009Article
- Mutation of a conserved glycine in the SH1-SH2 helix affects the load-dependent kinetics of myosin.Biophysical journal · 2007Article
- Imaging of cell migration.The EMBO journal · 2006Review
- A dibasic motif in the tail of a class XIV apicomplexan myosin is an essential determinant of plasma membrane localization.Molecular biology of the cell · 2000Article
- Article
- Switches, latches, and amplifiers: common themes of G proteins and molecular motors.The Journal of cell biology · 1996Review
- Structure-function studies of the myosin motor domain: importance of the 50-kDa cleft.Molecular biology of the cell · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Dictyostelium provides a powerful environment for characterization of myosin II function. It provides well-established biochemical methods for in vitro analysis of myosin's properties as well as an array of molecular genetic tools. The absence of myosin function results in an array of phenotypes that can be used to genetically manipulate myosin function. We have previously reported methods for the isolation and identification of rapid-effect cold-sensitive myosin II mutations in Dictyostelium. Here, we report the development and utilization of a rapid method for localizing these point mutations. We have also sequenced 19 mutants. The mutations show distinct clustering with respect to three-dimensional location and biochemically characterized functional domains of the protein. We conclude that these mutants represent powerful tools for understanding the mechanisms driving this protein motor.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.