ArticleGenetics1996
Meiotic recombination initiated by a double-strand break in rad50 delta yeast cells otherwise unable to initiate meiotic recombination.
Article in Genetics, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 61 citations in OpenAlex.
- Light-controlled Spo11-less meiotic DNA breaks by MagTAQing lead to chromosomal aberrations.Nucleic acids research · 2025Article
- Meiosis in budding yeast.Genetics · 2023Article
- Identification of the nuclear localization signal in the Saccharomyces cerevisiae Pif1 DNA helicase.PLoS genetics · 2023Article
- Single-strand template repair: key insights to increase the efficiency of gene editing.Current genetics · 2021Review
- A Rad51-independent pathway promotes single-strand template repair in gene editing.PLoS genetics · 2020Article
- HO Endonuclease-Initiated Recombination in Yeast Meiosis Fails To Promote Homologous Centromere Pairing and Is Not Constrained To Utilize the Dmc1 Recombinase.G3 (Bethesda, Md.) · 2018Article
- The chromokinesin Klp3a and microtubules facilitate acentric chromosome segregation.The Journal of cell biology · 2017Article
- Review
- The meiotic checkpoint network: step-by-step through meiotic prophase.Cold Spring Harbor perspectives in biology · 2014Review
- High throughput sequencing reveals alterations in the recombination signatures with diminishing Spo11 activity.PLoS genetics · 2013Article
- Coincident resection at both ends of random, γ-induced double-strand breaks requires MRX (MRN), Sae2 (Ctp1), and Mre11-nuclease.PLoS genetics · 2013Article
- Article
- Budding yeast Pch2, a widely conserved meiotic protein, is involved in the initiation of meiotic recombination.PloS one · 2012Article
- Pch2 modulates chromatid partner choice during meiotic double-strand break repair in Saccharomyces cerevisiae.Genetics · 2011Article
- Role of the Saccharomyces cerevisiae Rad53 checkpoint kinase in signaling double-strand breaks during the meiotic cell cycle.Molecular and cellular biology · 2008Article
- Biochemistry of Meiotic Recombination: Formation, Processing, and Resolution of Recombination Intermediates.Genome dynamics and stability · 2008Article
- Phosphorylation of the SQ H2A.X motif is required for proper meiosis and mitosis in Tetrahymena thermophila.Molecular and cellular biology · 2007Article
- Gene conversion and crossing over along the 405-kb left arm of Saccharomyces cerevisiae chromosome VII.Genetics · 2004Article
- Role of DNA replication proteins in double-strand break-induced recombination in Saccharomyces cerevisiae.Molecular and cellular biology · 2004Article
- The functions of budding yeast Sae2 in the DNA damage response require Mec1- and Tel1-dependent phosphorylation.Molecular and cellular biology · 2004Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Meiotic recombination in Saccharomyces cerevisiae is initiated by double- strand breaks (DSBs). We have developed a system to compare the properties of meiotic DSBs with those created by the site-specific HO endonuclease. HO endonuclease was expressed under the control of the meiotic-specific SPO13 promoter, creating a DSB at a single site on one of yeast's 16 chromosomes. In Rad+ strains the times of appearance of the HO-induced DSBs and of subsequent recombinants are coincident with those induced by normal meiotic DSBs. Physical monitoring of DNA showed that SPO13: : HO induced gene conversions both in Rad+ and in rad50 delta cells that cannot initiate normal meiotic DSBs. We find that the RAD50 gene is important, but not essential, for recombination even after a DSB has been created in a meiotic cell. In rad50 delta cells, some DSBs are not repaired until a broken chromosome has been packaged into a spore and is subsequently germinated. This suggests that a broken chromosome does not signal an arrest of progression through meiosis. The recombination defect in rad50 delta diploids is not, however, meiotic specific, as mitotic rad50 diploids, experiencing an HO-induced DSB, exhibit similar departures from wild-type recombination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.