ArticleThe Journal of general physiology1996
Ca2+ currents in cerebral artery smooth muscle cells of rat at physiological Ca2+ concentrations.
Article in The Journal of general physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed.
- Article
- A computational model predicts sex-specific responses to calcium channel blockers in mammalian mesenteric vascular smooth muscle.bioRxiv : the preprint server for biology · 2024Article
- Electrophysiological characterization of a schistosome transient receptor potential channel activated by praziquantel.International journal for parasitology · 2023Article
- Intraluminal pressure elevates intracellular calcium and contracts CNS pericytes: Role of voltage-dependent calcium channels.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Mechanisms and physiological implications of cooperative gating of clustered ion channels.Physiological reviews · 2022Review
- Transient receptor potential channel-dependent myogenic responsiveness in small-sized resistance arteries.Journal of exercise rehabilitation · 2021Review
- β-adrenergic-mediated dynamic augmentation of sarcolemmal CaThe Journal of physiology · 2019Article
- Reactive Oxygen Species Mediate the Suppression of Arterial Smooth Muscle T-type CaScientific reports · 2018Article
- Distance constraints on activation of TRPV4 channels by AKAP150-bound PKCα in arterial myocytes.The Journal of general physiology · 2017Article
- Sustained Contraction in Vascular Smooth Muscle by Activation of L-type CaFrontiers in pharmacology · 2016Article
- Location, Location, Location: Juxtaposed calcium-signaling microdomains as a novel model of the vascular smooth muscle myogenic response.The Journal of general physiology · 2015Article
- Membrane potential and Ca2+ concentration dependence on pressure and vasoactive agents in arterial smooth muscle: A model.The Journal of general physiology · 2015Article
- Orchestrating Ca2+ influx through Ca(V)1.2 and Ca(V)3.x channels in human cerebral arteries.The Journal of general physiology · 2015Article
- Vascular inward rectifier K+ channels as external K+ sensors in the control of cerebral blood flow.Microcirculation (New York, N.Y. : 1994) · 2015Review
- Chronic exercise normalizes changes in Cav 1.2 and KCa 1.1 channels in mesenteric arteries from spontaneously hypertensive rats.British journal of pharmacology · 2015Article
- Gap junctions suppress electrical but not [Ca(2+)] heterogeneity in resistance arteries.Biophysical journal · 2014Article
- Vascular TRP channels: performing under pressure and going with the flow.Physiology (Bethesda, Md.) · 2014Review
- Local control of TRPV4 channels by AKAP150-targeted PKC in arterial smooth muscle.The Journal of general physiology · 2014Article
- L-type Ca2+ channel sparklets revealed by TIRF microscopy in mouse urinary bladder smooth muscle.PloS one · 2014Article
- Down-regulation of CaV1.2 channels during hypertension: how fewer CaV1.2 channels allow more Ca(2+) into hypertensive arterial smooth muscle.The Journal of physiology · 2013Article
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Authors and funding
3 authors.
Funding
Abstract
Single Ca2+ channel and whole cell currents were measured in smooth muscle cells dissociated from resistance-sized (100-microns diameter) rat cerebral arteries. We sought to quantify the magnitude of Ca2+ channel currents and activity under the putative physiological conditions of these cells: 2 mM [Ca2+]o, steady depolarizations to potentials between -50 and -20 mV, and (where possible) without extrinsic channel agonists. Single Ca2+ channel conductance was measured over a broad range of Ca2+ concentrations (0.5-80 mM). The saturating conductance ranged from 1.5 pS at 0.5 mM to 7.8 pS at 80 mM, with a value of 3.5 pS at 2 mM Ca (unitary currents of 0.18 pA at -40 mV). Both single channel and whole cell Ca2+ currents were measured during pulses and at steady holding potentials. Ca2+ channel open probability and the lower limit for the total number of channels per cell were estimated by dividing the whole-cell Ca2+ currents by the single channel current. We estimate that an average cell has at least 5,000 functional channels with open probabilities of 3.4 x 10(-4) and 2 x 10(-3) at -40 and -20 mV, respectively. An average of 1-10 (-40 mV and -20 mV, respectively) Ca2+ channels are thus open at physiological potentials, carrying approximately 0.5 pA steady Ca2+ current at -30 mV. We also observed a very slow reduction in open probability during steady test potentials when compared with peak pulse responses. This 4-10-fold reduction in activity could not be accounted for by the channel's normal inactivation at our recording potentials between -50 and -20 mV, implying that an additional slow inactivation process may be important in regulating Ca2+ channel activity during steady depolarization.
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