ArticleThe Plant cell1996
Transport of DNA into the nuclei of xenopus oocytes by a modified VirE2 protein of Agrobacterium.
Article in The Plant cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Article
- Direct visualization of Agrobacterium-delivered VirE2 in recipient cells.The Plant journal : for cell and molecular biology · 2014Article
- Applying horizontal gene transfer phenomena to enhance non-viral gene therapy.Journal of controlled release : official journal of the Controlled Release Society · 2013Review
- Crystal structure of rice importin-α and structural basis of its interaction with plant-specific nuclear localization signals.The Plant cell · 2012Article
- Nuclear targeting of a bacterial integrase that mediates site-specific recombination between bacterial and human target sequences.Applied and environmental microbiology · 2011Article
- Agrobacterium tumefaciens and A. rhizogenes use different proteins to transport bacterial DNA into the plant cell nucleus.Microbial biotechnology · 2009Review
- IMPa-4, an Arabidopsis importin alpha isoform, is preferentially involved in agrobacterium-mediated plant transformation.The Plant cell · 2008Article
- Yeast-plant coupled vector system for identification of nuclear proteins.Plant physiology · 2007Article
- Arabidopsis VIRE2 INTERACTING PROTEIN2 is required for Agrobacterium T-DNA integration in plants.The Plant cell · 2007Article
- Nuclear localization signal peptides induce molecular delivery along microtubules.Biophysical journal · 2005Article
- The VirE3 protein of Agrobacterium mimics a host cell function required for plant genetic transformation.The EMBO journal · 2005Article
- Expression of plant protein phosphatase 2C interferes with nuclear import of the Agrobacterium T-complex protein VirD2.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- The VirD2 pilot protein of Agrobacterium-transferred DNA interacts with the TATA box-binding protein and a nuclear protein kinase in plants.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Agrobacterium-mediated plant transformation: the biology behind the "gene-jockeying" tool.Microbiology and molecular biology reviews : MMBR · 2003Review
- Analysis of Vir protein translocation from Agrobacterium tumefaciens using Saccharomyces cerevisiae as a model: evidence for transport of a novel effector protein VirE3.Nucleic acids research · 2003Article
- VIP1, an Arabidopsis protein that interacts with Agrobacterium VirE2, is involved in VirE2 nuclear import and Agrobacterium infectivity.The EMBO journal · 2001Article
- Genetic transformation of HeLa cells by Agrobacterium.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Import of Agrobacterium T-DNA into plant nuclei: two distinct functions of VirD2 and VirE2 proteins.The Plant cell · 2001Article
- The six functions of Agrobacterium VirE2.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Import of DNA into mammalian nuclei by proteins originating from a plant pathogenic bacterium.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We used Agrobacterium T-DNA nuclear transport to examine the specificity of nuclear targeting between plants and animals and the nuclear import of DNA by a specialized transport protein. Two karyophilic Agrobacterium virulence (Vir) proteins, VirD2 and VirE2, which presumably associate with the transported T-DNA and function in many plant species, were microinjected into Drosophila embryos and Xenopus oocytes. In both animal systems, VirD2 localized to the cell nuclei and VirE2 remained exclusively cytoplasmic, suggesting that VirE2 nuclear localization signals may be plant specific. Repositioning one amino acid residue within VirE2 nuclear localization signals enabled them to function in animal cells. The modified VirE2 protein bound DNA and actively transported it into the nuclei of Xenopus oocytes. These observations suggest a functional difference in nuclear import between animals and plants and show that DNA can be transported into the cell nucleus via a protein-specific pathway.
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