ArticleThe Biochemical journal1996
Structural changes in subdomain 2 of G-actin observed by fluorescence spectroscopy.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Myopathy-Sensitive G-Actin Segment 227-235 Is Involved in Salt-Induced Stabilization of Contacts within the Actin Filament.International journal of molecular sciences · 2021Article
- Nucleotide regulation of the structure and dynamics of G-actin.Biophysical journal · 2014Article
- Mutation of actin Tyr-53 alters the conformations of the DNase I-binding loop and the nucleotide-binding cleft.The Journal of biological chemistry · 2010Article
- F-actin structure destabilization and DNase I binding loop: fluctuations mutational cross-linking and electron microscopy analysis of loop states and effects on F-actin.Journal of molecular biology · 2010Article
- Effect of actin C-terminal modification on tropomyosin isoforms binding and thin filament regulation.Biochimica et biophysica acta · 2009Article
- Dual roles of Gln137 of actin revealed by recombinant human cardiac muscle alpha-actin mutants.The Journal of biological chemistry · 2008Article
- Role of actin C-terminus in regulation of striated muscle thin filament.Biophysical journal · 2008Article
- Allostery of actin filaments: molecular dynamics simulations and coarse-grained analysis.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The structure of nonvertebrate actin: implications for the ATP hydrolytic mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Cofilin and DNase I affect the conformation of the small domain of actin.Biophysical journal · 2002Article
- Role of the DNase-I-binding loop in dynamic properties of actin filament.Biophysical journal · 2002Article
- Neutrophil cytoskeletal disease.International journal of hematology · 2001Review
- Thymosin-beta(4) changes the conformation and dynamics of actin monomers.Biophysical journal · 2000Article
- A heterozygous mutation of beta-actin associated with neutrophil dysfunction and recurrent infection.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Divalent cation-, nucleotide-, and polymerization-dependent changes in the conformation of subdomain 2 of actin.Biophysical journal · 1999Article
- A change in actin conformation associated with filament instability after Pi release.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Differential epitope tagging of actin in transformed Drosophila produces distinct effects on myofibril assembly and function of the indirect flight muscle.Molecular biology of the cell · 1999Article
- Localization of a single transglutaminase-reactive glutamine in the third domain of RAP, the alpha2-macroglobulin receptor-associated protein.Journal of protein chemistry · 1999Article
- Effect of Ca2+-Mg2+ exchange on the flexibility and/or conformation of the small domain in monomeric actin.Biophysical journal · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The influence of DNase I binding to Ca-ATP-G-actin and of Ca2+/Mg2+ and ATP/ADP exchange on the conformation of G-actin were investigated by measuring the fluorescence of dansyl cadaverine (DC) conjugated to Gln41 in subdomain 2 of the protein. Fluorescence resonance energy transfer (FRET) between this probe and N-[4-(dimethylamino)-3,5-dinitrophenyl]maleimide (DDPM) attached to Cys374 in subdomain 1 was also measured. Contrary to an earlier report [dos Remedios, Kiessling and Hambly (1994) in Synchrotron Radiation in the Biosciences (Chance, B., Deisenhofer, J., Ebashi, S., Goodhead, D. T., Helliwell, J. R., Huxley, H. E., Iizuka, T., Kirz, J., Mitsui, T., Rubenstein, E. et al., eds.), pp. 418-425, Oxford University Press, Oxford], the distance between these probes did not change significantly when DNase I was bound to actin. A small but reproducible increase in the quantum yield and a blue shift of the DC fluorescence maximum were observed when bound Ca2+ was replaced by Mg2+. A large increase (about 70%) in the quantum yield and an approx. 12 nm blue shift of the emission spectrum occurred when ATP in Mg-G-actin was replaced by ADP. These changes were not accompanied by any significant change in the FRET distance between the dansyl donor and DDPM acceptor probes. A substantial change in the fluorescence of DC-actin was observed after proteolytic removal of the last three residues of actin, in accordance with earlier evidence suggesting that there is a conformational coupling between subdomain 2 and the C-terminal segment in subdomain 1 of actin. The results are discussed in relation to recently published data obtained with another fluorescent probe and to earlier observations based on limited cleavage using proteolytic enzymes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.