ArticleThe Biochemical journal1996
Characterization of the effects of Ca2+ depletion on the synthesis, phosphorylation and secretion of caseins in lactating mammary epithelial cells.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 44 citations in OpenAlex.
- The Trans Golgi Region is a Labile Intracellular CaScientific reports · 2018Article
- Nutrient transport in the mammary gland: calcium, trace minerals and water soluble vitamins.Journal of mammary gland biology and neoplasia · 2014Review
- Intracellular organelles in the saga of Ca2+ homeostasis: different molecules for different purposes?Cellular and molecular life sciences : CMLS · 2012Review
- The trans-golgi compartment: A new distinct intracellular Ca store.Communicative & integrative biology · 2010Article
- Unique characteristics of Ca2+ homeostasis of the trans-Golgi compartment.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Calnuc plays a role in dynamic distribution of Galphai but not Gbeta subunits and modulates ACTH secretion in AtT-20 neuroendocrine secretory cells.Molecular neurodegeneration · 2009Article
- Nonsense-mediated decay of human HEXA mRNA.Molecular and cellular biology · 2001Article
- Purification of Golgi casein kinase from bovine milk.The Biochemical journal · 2000Article
- Calnuc, an EF-hand Ca(2+) binding protein, specifically interacts with the C-terminal alpha5-helix of G(alpha)i3.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Overexpression of CALNUC (nucleobindin) increases agonist and thapsigargin releasable Ca2+ storage in the Golgi.The Journal of cell biology · 1999Article
- The Golgi apparatus is an inositol 1,4,5-trisphosphate-sensitive Ca2+ store, with functional properties distinct from those of the endoplasmic reticulum.The EMBO journal · 1998Article
- Mammary gland membrane transport systems.Journal of mammary gland biology and neoplasia · 1998Review
- Secretion of milk proteins.Journal of mammary gland biology and neoplasia · 1998Review
- Calcium and vitamin D metabolism during lactation.Journal of mammary gland biology and neoplasia · 1997Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have examined the effects of depleting lumenal Ca2+ on the synthesis, phosphorylation and secretion of caseins in lactating mouse mammary cells by using inhibitors of the endoplasmic reticulum Ca(2+)-ATPase or the ionophore ionomycin in the absence of external Ca2+. Treatment with these drugs resulted in a transient increase in the cytosolic Ca2+ concentration due to Ca2+ mobilization. Protein synthesis over a 1 h period was substantially inhibited by Ca2+ depletion, but in a pulse-chase protocol secretion of pre-synthesized proteins was unaffected by Ca2+ depletion. Analysis of polysome profiles showed that Ca2+ depletion resulted in a loss of polysomes, consistent with an inhibition of initiation of protein synthesis. Neither treatment with Ca(2+)-ATPase inhibitors to deplete endoplasmic reticulum Ca2+ nor treatment with ionomycin/EGTA had any effect on an early phase of phosphorylation of alpha- or beta/gamma-caseins, but Ca2+ depletion resulted in a decrease in a late phase of casein phosphorylation. These results indicate that lumenal Ca2+ is required to maintain protein synthesis in lactating mammary cells but is not required for protein secretion, and that Ca2+ accumulation in the Golgi cisternae is required for a late but not for an early phase of casein phosphorylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.