ArticleThe Biochemical journal1996
Ca2+ signalling mechanisms of the beta 2 integrin on neutrophils: involvement of phospholipase C gamma 2 and Ins(1,4,5)P3.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 81 citations in OpenAlex.
- Neutrophil Mechanosignaling Promotes Integrin Engagement With Endothelial Cells and Motility Within Inflamed Vessels.Frontiers in immunology · 2018Trial
- Review
- Localisation of Intracellular Signals and Responses during Phagocytosis.International journal of molecular sciences · 2023Review
- PLCγ2 regulates TREM2 signalling and integrin-mediated adhesion and migration of human iPSC-derived macrophages.Scientific reports · 2021Article
- Cellular Mechanisms of NETosis.Annual review of cell and developmental biology · 2020Review
- Monoclonal Antibody to CD14, TLR4, or CD11b: Impact of Epitope and Isotype Specificity on ROS Generation by Human Granulocytes and Monocytes.Oxidative medicine and cellular longevity · 2020Review
- Neutrophil Cell Shape Change: Mechanism and Signalling during Cell Spreading and Phagocytosis.International journal of molecular sciences · 2019Review
- U-73122 reduces the cell growth in cultured MG-63 ostesarcoma cell line involving Phosphoinositide-specific Phospholipases C.SpringerPlus · 2016Article
- Role of the store-operated calcium entry protein, STIM1, in neutrophil chemotaxis and infiltration into a murine model of psoriasis-inflamed skin.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015Article
- Leukocyte function antigen-1, kindlin-3, and calcium flux orchestrate neutrophil recruitment during inflammation.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Chemokines, selectins and intracellular calcium flux: temporal and spatial cues for leukocyte arrest.Frontiers in immunology · 2012Article
- Physiological and pathophysiological functions of SOCE in the immune system.Frontiers in bioscience (Elite edition) · 2012Review
- β2 integrin induces TCRζ-Syk-phospholipase C-γ phosphorylation and paxillin-dependent granule polarization in human NK cells.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- The phospholipase C inhibitor U-73122 inhibits Ca(2+) release from the intracellular sarcoplasmic reticulum Ca(2+) store by inhibiting Ca(2+) pumps in smooth muscle.British journal of pharmacology · 2010Article
- Article
- Regulation of superoxide production in neutrophils: role of calcium influx.Journal of leukocyte biology · 2008Review
- Phospholipase Cgamma2 modulates integrin signaling in the osteoclast by affecting the localization and activation of Src kinase.Molecular and cellular biology · 2008Article
- Calcium flux in neutrophils synchronizes beta2 integrin adhesive and signaling events that guide inflammatory recruitment.Annals of biomedical engineering · 2008Article
- Vav family proteins are required for optimal regulation of PLCgamma2 by integrin alphaIIbbeta3.The Biochemical journal · 2007Article
- Calreticulin couples calcium release and calcium influx in integrin-mediated calcium signaling.Molecular biology of the cell · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Engagement of beta 2 integrins triggers a tyrosine kinase-dependent intracellular mobilization and influx of Ca2+ in human neutrophils. However, the transduction pathway involved in generating this Ca2+ signal is obscure. In the present study we identified phospholipase C gamma 2 (PLC gamma 2) as one of the major proteins that was phosphorylated on tyrosine in response to beta 2 integrin activation. This beta 2 integrin-induced phosphorylation of PLC gamma 2 occurred in parallel with an increased accumulation of Ins(1,4,5)P3. The relevance of these observations for the beta 2 integrin-induced Ca2+ signal was investigated using an inhibitor of PLC signalling pathways, 1-(6-{[17 beta-3-methoxyoestra-1,3.5(10)-trien-17-yl] amino}hexyl)-1H-pyrrole-2,5-dione(U73122). U73122 dose-dependently (IC50, approx. 0.15 microM) inhibited both the beta 2 integrin-induced release of Ca2+ from intracellular stores and the subsequent influx of Ca2+ across the plasma membrane. These effects were not observed with the inactive analogue 1-(6-{[17 beta-3-methoxyoestra-1,3,5(10)-trien-17-yl] amino}hexyl)-pyrrolidine-2,5-dione (U73343). To gain further support for an involvement of PLC-induced Ins(1,4,5)P3 formation in the beta 2 integrin-induced Ca2+ signal, we searched for the molecular event(s) underlying the effects of U73122. Our experiments revealed that U73122 had no effect on either beta 2 integrin-induced tyrosine phosphorylation of PLC gamma 2 (or any of the other proteins) or on the formation of Ins(1,4,5)P3, but it reduced the Ins(1,4,5)P3-induced release of 45Ca2+ from intracellular stores of electropermeabilized cells. Taken together, the present data suggest that the beta 2 integrin-induced Ca2+ signal in human neutrophils is generated through activation of a PLC gamma 2-dependent pathway.
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