ArticleProceedings of the National Academy of Sciences of the United States of America1996
Overexpression of the transcription factor UBF1 is sufficient to increase ribosomal DNA transcription in neonatal cardiomyocytes: implications for cardiac hypertrophy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 66 citations in OpenAlex.
- Article
- Sex-specific effects ofJournal of molecular and cellular cardiology plus · 2023Article
- Ribosome accumulation during early phase resistance training in humans.Acta physiologica (Oxford, England) · 2022Article
- NAP1L5 Promotes Nucleolar Hypertrophy and Is Required for Translation Activation During Cardiomyocyte Hypertrophy.Frontiers in cardiovascular medicine · 2021Article
- African trypanosomes expressing multiple VSGs are rapidly eliminated by the host immune system.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Heterozygous De Novo UBTF Gain-of-Function Variant Is Associated with Neurodegeneration in Childhood.American journal of human genetics · 2017Article
- Attenuation of microRNA-16 derepresses the cyclins D1, D2 and E1 to provoke cardiomyocyte hypertrophy.Journal of cellular and molecular medicine · 2015Article
- Ribosome abundance regulates the recovery of skeletal muscle protein mass upon recuperation from postnatal undernutrition in mice.The Journal of physiology · 2014Article
- Genome-wide microRNA changes in human intracranial aneurysms.BMC neurology · 2014Article
- Ribosome biogenesis in skeletal development and the pathogenesis of skeletal disorders.Biochimica et biophysica acta · 2014Review
- Nucleolar dysfunction in Huntington's disease.Biochimica et biophysica acta · 2014Review
- ESET methylates UBF at K232/254 and regulates nucleolar heterochromatin plasticity and rDNA transcription.Nucleic acids research · 2014Article
- Dysregulation of upstream binding factor-1 acetylation at K352 is linked to impaired ribosomal DNA transcription in Huntington's disease.Cell death and differentiation · 2011Article
- Ribosomal biogenesis induction by high glucose requires activation of upstream binding factor in kidney glomerular epithelial cells.American journal of physiology. Renal physiology · 2011Article
- UBF levels determine the number of active ribosomal RNA genes in mammals.The Journal of cell biology · 2008Article
- Helix-loop-helix protein p8, a transcriptional regulator required for cardiomyocyte hypertrophy and cardiac fibroblast matrix metalloprotease induction.Molecular and cellular biology · 2007Article
- Direct regulation of rRNA transcription by fibroblast growth factor 2.Molecular and cellular biology · 2005Article
- MAD1 and c-MYC regulate UBF and rDNA transcription during granulocyte differentiation.The EMBO journal · 2004Article
- Putative involvement of the histone acetyltransferase Tip60 in ribosomal gene transcription.Nucleic acids research · 2004Article
- mTOR-dependent regulation of ribosomal gene transcription requires S6K1 and is mediated by phosphorylation of the carboxy-terminal activation domain of the nucleolar transcription factor UBF.Molecular and cellular biology · 2003Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
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Abstract
The accelerated protein accumulation characteristic of cardiomyocyte hypertrophy results from increased cellular protein synthetic capacity (elevated ribosome content). The rate limiting step in ribosome accumulation is transcription of the rRNA genes. During neonatal cardiomyocyte hypertrophy induced by norepinephrine or spontaneous contraction, changes in the expression of a ribosomal DNA transcription factor, UBF, correlated with increased rates of ribosome biogenesis. We hypothesized that elevated expression of UBF was part of the mechanism by which these hypertrophic stimuli effected increases in the rate of transcription from the rDNA promoter. In this study, we have examined directly the effect of overexpressing UBF on rDNA transcription in neonatal cardiomyocytes in culture. In control experiments, a novel reporter construct for rDNA transcription (pSMECAT) showed similar increases in activity in response to hypertrophic stimuli (10(-4) M phenylephrine, 10(-7) M endothelin, and spontaneous contraction) as did the endogenous rRNA genes. When contraction-arrested cardiomyocytes were cotransfected with pSMECAT and increasing amounts of a UBF1 expression vector; a dose-dependent (3-5 fold) increase in rDNA transcription was observed. Western blot analysis confirmed that the overexpressed, FLAG-tagged UBF accumulated in the cardiomyocyte nuclei. The observation that overexpression of UBF1 is sufficient to increase rDNA transcription in neonatal cardiomyocytes provides evidence in support of the hypothesis that the regulation of UBF is a key component of the increased ribosome biogenesis and protein accumulation associated with cardiomyocyte hypertrophy.
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