ArticleProceedings of the National Academy of Sciences of the United States of America1996
Circulating plasma xanthine oxidase contributes to vascular dysfunction in hypercholesterolemic rabbits.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 372 citations in OpenAlex.
- Superoxide-mediated inactivation of nitric oxide and peroxynitrite formation by tobacco smoke in vascular endothelium: studies in cultured cells and smokers.American journal of physiology. Heart and circulatory physiology · 2009Trial
- Oxypurinol improves coronary and peripheral endothelial function in patients with coronary artery disease.Free radical biology & medicine · 2005Trial
- Regulation of nitric oxide generation and consumption.International journal of biological sciences · 2025Review
- Association of plasma xanthine oxidoreductase activity with vascular endothelial function independent of serum uric acid level: MedCity21 health examination registry.International journal of cardiology. Heart & vasculature · 2023Article
- Antioxidant Properties of Oral Antithrombotic Therapies in Atherosclerotic Disease and Atrial Fibrillation.Antioxidants (Basel, Switzerland) · 2023Review
- Effects of superoxide anion attack on the lipoprotein HDL.Molecular and cellular biochemistry · 2023Article
- Hyperoxaluria Induces Endothelial Dysfunction in Preglomerular Arteries: Involvement of Oxidative Stress.Cells · 2022Article
- Article
- A Multiple-Hit Hypothesis Involving Reactive Oxygen Species and Myeloperoxidase Explains Clinical Deterioration and Fatality in COVID-19.International journal of biological sciences · 2021Review
- Superoxide-induced Type I collagen secretion depends on prolyl 4-hydroxylases.Biochemical and biophysical research communications · 2020Article
- S-Nitrosylation: An Emerging Paradigm of Redox Signaling.Antioxidants (Basel, Switzerland) · 2019Review
- Plasma xanthine oxidase activity is related to increased sodium and left ventricular hypertrophy in resistant hypertension.Free radical biology & medicine · 2019Article
- The impact of xanthine oxidase (XO) on hemolytic diseases.Redox biology · 2019Review
- Activity of xanthine oxidase in plasma correlates with indices of insulin resistance and liver dysfunction in patients with type 2 diabetes mellitus and metabolic syndrome: A pilot exploratory study.Journal of diabetes investigation · 2019Article
- Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their RegulationPhysiological reviews · 2019Review
- A magnetic resonance multi-atlas for the neonatal rabbit brain.NeuroImage · 2018Article
- Side Effects and Interactions of the Xanthine Oxidase Inhibitor Febuxostat.Pharmaceuticals (Basel, Switzerland) · 2018Review
- Xanthine oxidase activity in patients with age-related cataract associated with hypertension.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2018Article
- Arctic ground squirrel resist peroxynitrite-mediated cell death in response to oxygen glucose deprivation.Free radical biology & medicine · 2017Article
- The Future Challenge of Reactive Oxygen Species (ROS) in Hypertension: From Bench to Bed Side.International journal of molecular sciences · 2017Review
15 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Reactive oxygen species play a central role in vascular inflammation and atherogenesis, with enhanced superoxide (O2.-) production contributing significantly to impairment of nitric oxide (.NO)-dependent relaxation of vessels from cholesterol-fed rabbits. We investigated potential sources of O2.- production, which contribute to this loss of endothelium-dependent vascular responses. The vasorelaxation elicited by acetylcholine (ACh) in phenylephrine-contracted, aortic ring segments was impaired by cholesterol feeding. Pretreatment of aortic vessels with either heparin, which competes with xanthine oxidase (XO) for binding to sulfated glycosaminoglycans, or the XO inhibitor allopurinol resulted in a partial restoration (36-40% at 1 muM ACh) of ACh-dependent relaxation. Furthermore, O2.(-)-dependent lucigenin chemiluminescence, measured in intact ring segments from hypercholesterolemic rabbits, was decreased by addition of heparin, allopurinol or a chimeric, heparin-binding superoxide dismutase. XO activity was elevated more than two-fold in plasma of hypercholesterolemic rabbits. Incubation of vascular rings from rabbits on a normal diet with purified XO (10 milliunits/ml) also impaired .NO-dependent relaxation but only in the presence of purine substrate. As with vessels from hypercholesterolemic rabbits, this effect was prevented by heparin and allopurinol treatment. We hypothesize that increases in plasma cholesterol induce the release of XO into the circulation, where it binds to endothelial cell glycosaminoglycans. Only in hypercholesterolemic vessels is sufficient substrate available to sustain the production of O2.- and impair NO-dependent vasorelaxation. Chronically, the continued production of peroxynitrite, (ONOO-) which the simultaneous generation of NO and O2.- implies, may irreversibly impair vessel function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.