ArticleProceedings of the National Academy of Sciences of the United States of America1996
Antisense ribosomes: rRNA as a vehicle for antisense RNAs.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Personalized mRNA vaccines in glioblastoma therapy: from rational design to clinical trials.Journal of nanobiotechnology · 2024Review
- Article
- mRNA vaccines: A novel weapon to control infectious diseases.Frontiers in microbiology · 2022Review
- Review
- Homoiterons and expansion in ribosomal RNAs.FEBS open bio · 2015Article
- An overview of nested genes in eukaryotic genomes.Eukaryotic cell · 2009Review
- Nested genes CDA12 and CDA13 encode proteins associated with membrane trafficking in the ciliate Tetrahymena thermophila.Eukaryotic cell · 2009Article
- Induction of gene silencing by hairpin RNA expression in Tetrahymena thermophila reveals a second small RNA pathway.Molecular and cellular biology · 2006Article
- The bifunctional dihydrofolate reductase thymidylate synthase of Tetrahymena thermophila provides a tool for molecular and biotechnology applications.BMC biotechnology · 2006Article
- Genetic, genomic, and functional analysis of the granule lattice proteins in Tetrahymena secretory granules.Molecular biology of the cell · 2005Article
- Diffusion-based transport of nascent ribosomes in the nucleus.Molecular biology of the cell · 2003Article
- The ribosome filter hypothesis.Proceedings of the National Academy of Sciences of the United States of America · 2002Review
- Untranslated regions of mRNAs.Genome biology · 2002Review
- An antisense approach to phenotype-based gene cloning in Tetrahymena.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Complementarity between the mRNA 5' untranslated region and 18S ribosomal RNA can inhibit translation.RNA (New York, N.Y.) · 2000Article
- rRNA-complementarity in the 5' untranslated region of mRNA specifying the Gtx homeodomain protein: evidence that base- pairing to 18S rRNA affects translational efficiency.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- rRNA complementarity within mRNAs: a possible basis for mRNA-ribosome interactions and translational control.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Proteolytic processing and Ca2+-binding activity of dense-core vesicle polypeptides in Tetrahymena.Molecular biology of the cell · 1998Article
- Tetrahymena: the key to the genetic analysis of the regulated pathway of polypeptide secretion?Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- rRNA-like sequences occur in diverse primary transcripts: implications for the control of gene expression.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although rRNA has a conserved core structure, its size varies by more than 2000 bases between eubacteria and vertebrates, mostly due to the size variation of discrete variable regions. Previous studies have shown that insertion of foreign sequences into some of these variable regions has little effect on rRNA function. These properties make rRNA a potentially very advantageous vehicle to carry other RNA moieties with biological activity, such as "antisense RNAs." We have explored this possibility by inserting antisense RNAs targeted against one essential and two nonessential genes into a site within a variable region in the Tetrahymena thermophila large subunit rRNA gene. Expression of each of the three genes tested can be drastically reduced or eliminated in transformed T. thermophila lines containing these altered rRNAs. In addition, we found that only antisense rRNAs containing RNA sequences complementary to the 5' untranslated region of the targeted mRNA were effective. Lines containing antisense rRNAs targeted against either of the nonessential genes grow well, indicating that the altered rRNAs fulfill their functions within the ribosome. Since functional rRNA is extremely abundant and stable and comes into direct contact with translated mRNAs, it may prove to be an unparalleled vehicle for enhancing the activity of functional RNAs that act on mRNAs.
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