ArticleProceedings of the National Academy of Sciences of the United States of America1996
Sequence tag identification of intact proteins by matching tanden mass spectral data against sequence data bases.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 220 citations in OpenAlex.
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- Accurate and Efficient Resolution of Overlapping Isotopic Envelopes in Protein Tandem Mass Spectra.Scientific reports · 2015Article
- A peptide identification-free, genome sequence-independent shotgun proteomics workflow for strain-level bacterial differentiation.Scientific reports · 2015Article
- Characterization of green fluorescent proteins by 193 nm ultraviolet photodissociation mass spectrometry.Proteomics · 2014Article
- Protein-protein interactions: switch from classical methods to proteomics and bioinformatics-based approaches.Cellular and molecular life sciences : CMLS · 2014Review
- Integral membrane proteins and bilayer proteomics.Analytical chemistry · 2013Review
- Analysis of naphthalene adduct binding sites in model proteins by tandem mass spectrometry.Chemico-biological interactions · 2012Article
- Profiling of integral membrane proteins and their post translational modifications using high-resolution mass spectrometry.Methods (San Diego, Calif.) · 2011Review
- Top-Down Mass Spectrometry of Supercharged Native Protein-Ligand Complexes.International journal of mass spectrometry · 2011Article
- Native electrospray and electron-capture dissociation in FTICR mass spectrometry provide top-down sequencing of a protein component in an intact protein assembly.Journal of the American Society for Mass Spectrometry · 2010Article
- Reactive gamma-ketoaldehydes formed via the isoprostane pathway disrupt mitochondrial respiration and calcium homeostasis.Free radical biology & medicine · 2010Article
- Confident assignment of intact mass tags to human salivary cystatins using top-down Fourier-transform ion cyclotron resonance mass spectrometry.Journal of the American Society for Mass Spectrometry · 2010Article
- Gas-phase chemistry of multiply charged bioions in analytical mass spectrometry.Annual review of analytical chemistry (Palo Alto, Calif.) · 2010Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Molecular and fragment ion data of intact 8- to 43-kDa proteins from electrospray Fourier-transform tandem mass spectrometry are matched against the corresponding data in sequence data bases. Extending the sequence tag concept of Mann and Wilm for matching peptides, a partial amino acid sequence in the unknown is first identified from the mass differences of a series of fragment ions, and the mass position of this sequence is defined from molecular weight and the fragment ion masses. For three studied proteins, a single sequence tag retrieved only the correct protein from the data base; a fourth protein required the input of two sequence tags. However, three of the data base proteins differed by having an extra methionine or by missing an acetyl or heme substitution. The positions of these modifications in the protein examined were greatly restricted by the mass differences of its molecular and fragment ions versus those of the data base. To characterize the primary structure of an unknown represented in the data base, this method is fast and specific and does not require prior enzymatic or chemical degradation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.