ArticleJournal of virology1996
Characterization of a 120-Kilodalton pre-S-binding protein as a candidate duck hepatitis B virus receptor.
Article in Journal of virology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Primary Duck Hepatocyte Culture and Duck Hepatitis B Virus Infection Model.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Host receptors: the key to establishing cells with broad viral tropism for vaccine production.Critical reviews in microbiology · 2020Review
- From DCPD to NTCP: the long journey towards identifying a functional hepatitis B virus receptor.Clinical and molecular hepatology · 2015Review
- Virus entry mediated by hepatitis B virus envelope proteins.World journal of gastroenterology · 2013Review
- Hepatitis C virus NS2 protein triggers endoplasmic reticulum stress and suppresses its own viral replication.Journal of hepatology · 2010Article
- Initiation of duck hepatitis B virus infection requires cleavage by a furin-like protease.Journal of virology · 2010Article
- Hepatitis B virus-cell interactions and pathogenesis.Journal of cellular physiology · 2008Review
- Entry of duck hepatitis B virus into primary duck liver and kidney cells after discovery of a fusogenic region within the large surface protein.Journal of virology · 2007Article
- Viral and cellular determinants involved in hepadnaviral entry.World journal of gastroenterology · 2007Review
- Molecular characterization of duck hepatitis B virus isolated from Hubei brown ducks.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2006Article
- Mapping of the hepatitis B virus pre-S1 domain involved in receptor recognition.Journal of virology · 2005Article
- Glycine decarboxylase mediates a postbinding step in duck hepatitis B virus infection.Journal of virology · 2004Article
- Study of the early steps of the Hepatitis B Virus life cycle.International journal of medical sciences · 2004Article
- Evolution of cell recognition by viruses: a source of biological novelty with medical implications.Advances in virus research · 2003Review
- HBV infection of cell culture: evidence for multivalent and cooperative attachment.The EMBO journal · 2001Article
- Carboxypeptidase D is an avian hepatitis B virus receptor.Journal of virology · 1999Article
- Carboxypeptidase D (gp180), a Golgi-resident protein, functions in the attachment and entry of avian hepatitis B viruses.Journal of virology · 1998Article
- Enhancement of hepatitis B virus infection by noninfectious subviral particles.Journal of virology · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Infection by human and animal hepadnaviruses displays remarkable host and tissue tropism. The infection cycle probably initiates with binding of the pre-S domain of viral envelope protein to surface receptors present on the hepatocyte. Three types of neutralizing monoclonal antibodies against duck hepatitis B virus (DHBV) have their binding sites clustered within residues 83 to 107 of the pre-S protein, suggesting that this region may constitute a major receptor binding site. A 170- or 180-kDa duck protein (p170 or gp180) which binds DHBV particles through this part of the pre-S sequence has been identified recently. Although the p170 binding protein is host (duck) specific, its distribution is not restricted to DHBV-infectible tissues. Using the pre-S protein fused to glutathione S-transferase and immobilized on Sepharose beads, we have now identified an additional binding protein with a size of 120 kDa (p120). p120 expression is restricted to the liver, kidney, and pancreas, the three major organs of DHBV replication. While optimal p170 binding requires an intact pre-S protein, binding to p120 occurs much more efficiently with a few N- or C-terminally truncated forms. The p120 binding site was mapped to residues 98 to 102 of the pre-S region, which overlaps with a cluster of known virus-neutralizing epitopes. Site-directed mutagenesis revealed residues 100 to 102 (Phe-Arg-Arg) as the critical p120 contact site; nonconservative substitution in any of the three positions abolished p120 binding. Double mutations at positions 100 to 102 markedly reduced DHBV infectivity in cell culture. Short pre-S peptides covering the clustered neutralizing epitopes (also p170 and p120 binding sites) reduced DHBV infectivity in primary duck hepatocyte cultures. Thus, p120 represents a candidate component of the DHBV receptor complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.