ArticleThe Journal of cell biology1996
Altered rate of fibronectin matrix assembly by deletion of the first type III repeats.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 161 citations in OpenAlex.
- SNED1 modulates ECM architecture and cell proliferation via its LDV integrin-binding motif.Journal of cell science · 2026Article
- SNED1 fibrillar assembly in the extracellular matrix requires fibronectin and collagen I.Journal of cell science · 2026Article
- Hydrogel-Delivered Recombinant Fibronectin DK1 Promotes Diabetic Wound Healing by Boosting Cellular Responses.Biomolecules · 2026Article
- SNED1 modulates ECM architecture and cell proliferation via LDV-binding integrins.bioRxiv : the preprint server for biology · 2026Article
- SNED1 fibrillar assembly in the extracellular matrix requires fibronectin and collagen I.bioRxiv : the preprint server for biology · 2026Article
- Vinculin Y822 phosphorylation regulates adhesion remodeling during cardiomyocyte maturation.Journal of cell science · 2025Article
- Exosomes are specialized vehicles to induce fibronectin assembly.bioRxiv : the preprint server for biology · 2025Article
- Fibronectin matrix assembly at a glance.Journal of cell science · 2025Review
- SOCS domain targets ECM assembly in lung fibroblasts and experimental lung fibrosis.Scientific reports · 2024Article
- SOCS domain targets ECM assembly in lung fibroblasts and experimental lung fibrosis.bioRxiv : the preprint server for biology · 2024Article
- Actomyosin-mediated cellular tension promotes Yap nuclear translocation and myocardial proliferation through α5 integrin signaling.Development (Cambridge, England) · 2023Article
- Noncanonical Wnt/Ror2 signaling regulates cell-matrix adhesion to prompt directional tumor cell invasion in breast cancer.Molecular biology of the cell · 2022Article
- Review
- A new mechanism of fibronectin fibril assembly revealed by live imaging and super-resolution microscopy.Journal of cell science · 2022Article
- Heparan sulfate is necessary for the early formation of nascent fibronectin and collagen I fibrils at matrix assembly sites.The Journal of biological chemistry · 2022Article
- USP10 Promotes Fibronectin Recycling, Secretion, and Organization.Investigative ophthalmology & visual science · 2021Article
- Review
- A multilayered scaffold for regeneration of smooth muscle and connective tissue layers.Journal of biomedical materials research. Part A · 2021Article
- Fibronectin assembly regulates lumen formation in breast acini.Journal of cellular biochemistry · 2021Article
- Fibronectin as a multiregulatory molecule crucial in tumor matrisome: from structural and functional features to clinical practice in oncology.Journal of experimental & clinical cancer research : CR · 2021Review
15 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The assembly of fibronectin (FN) into a fibrillar matrix is a complex stepwise process that involves binding to integrin receptors as well as interactions between FN molecules. To follow the progression of matrix formation and determine the stages during which specific domains function, we have developed cell lines that lack an endogenous FN matrix but will form fibrils when provided with exogenous FN. Recombinant FNs (recFN) containing deletions of either the RGD cell-binding sequence (RGD-) or the first type III repeats (FN delta III1-7) including the III1 FN binding site were generated with the baculovirus insect cell expression system. After addition to cells, recFN matrix assembly was monitored by indirect immunofluorescence and by insolubility in the detergent deoxycholate (DOC). In the absence of any native FN, FN delta III1-7 was assembled into fibrils and was converted into DOC-insoluble matrix. This process could be inhibited by the amino-terminal 70 kD fragment of FN, showing that FN delta III1-7 follows an assembly pathway similar to FN. The progression of FN delta III1-7 assembly differed from native FN in that the recFN became DOC-insoluble more quickly. In contrast, RGD- recFNs were not formed into fibrils except when added in combination with native FN. These results show that the RGD sequence is essential for the initiation step but fibrils can form independently of the III1-7 modules. The altered rate of FN delta III1-7 assembly suggests that one function of the missing repeats might be to modulate an early stage of matrix formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.