ArticleThe Journal of cell biology1996
Analysis of Tub4p, a yeast gamma-tubulin-like protein: implications for microtubule-organizing center function.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
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Who cites it
75 citing papers in PubMed, 155 citations in OpenAlex.
- Spatial cues and not spindle pole maturation drive the asymmetry of astral microtubules between new and preexisting spindle poles.Molecular biology of the cell · 2018Article
- High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast.Journal of visualized experiments : JoVE · 2017Article
- Coordination of Cell Cycle Progression and Mitotic Spindle Assembly Involves Histone H3 Lysine 4 Methylation by Set1/COMPASS.Genetics · 2017Article
- Interaction of CK1δ with γTuSC ensures proper microtubule assembly and spindle positioning.Molecular biology of the cell · 2015Article
- Kinesin-14 and kinesin-5 antagonistically regulate microtubule nucleation by γ-TuRC in yeast and human cells.Nature communications · 2014Article
- Lessons from yeast: the spindle pole body and the centrosome.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Chemical suppression of defects in mitotic spindle assembly, redox control, and sterol biosynthesis by hydroxyurea.G3 (Bethesda, Md.) · 2014Article
- Distinct roles for antiparallel microtubule pairing and overlap during early spindle assembly.Molecular biology of the cell · 2013Article
- Fission yeast MOZART1/Mzt1 is an essential γ-tubulin complex component required for complex recruitment to the microtubule organizing center, but not its assembly.Molecular biology of the cell · 2013Article
- APJ1 and GRE3 homologs work in concert to allow growth in xylose in a natural Saccharomyces sensu stricto hybrid yeast.Genetics · 2012Article
- Constitutive dynein activity in She1 mutants reveals differences in microtubule attachment at the yeast spindle pole body.Molecular biology of the cell · 2012Article
- An extended γ-tubulin ring functions as a stable platform in microtubule nucleation.The Journal of cell biology · 2012Article
- Mitotic spindle form and function.Genetics · 2012Article
- Endoplasmic reticulum stress regulation of the Kar2p/BiP chaperone alleviates proteotoxicity via dual degradation pathways.Molecular biology of the cell · 2012Article
- Feeding cells induced by phytoparasitic nematodes require γ-tubulin ring complex for microtubule reorganization.PLoS pathogens · 2011Article
- Article
- Anastral spindle assembly and γ-tubulin in Drosophila oocytes.BMC cell biology · 2011Article
- Interplay of substrate retention and export signals in endoplasmic reticulum quality control.PloS one · 2010Article
- Mobility, microtubule nucleation and structure of microtubule-organizing centers in multinucleated hyphae of Ashbya gossypii.Molecular biology of the cell · 2010Article
- A multicomponent assembly pathway contributes to the formation of acentrosomal microtubule arrays in interphase Drosophila cells.Molecular biology of the cell · 2008Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
gamma-Tubulin is a conserved component of microtubule-organizing centers and is thought to be involved in microtubule nucleation. A recently discovered Saccharomyces cerevisiae gene (TUB4) encodes a tubulin that is related to, but divergent from, gamma-tubulins. TUB4 is essential for cell viability, and epitope-tagged Tub4 protein (Tub4p) is localized to the spindle pole body (Sobel, S.G., and M. Snyder. 1995.J. Cell Biol. 131:1775-1788). We have characterized the expression of TUB4, the association of Tub4p with the spindle pole body, and its role in microtubule organization. Tub4p is a minor protein in the cell, and expression of TUB4 is regulated in a cell cycle-dependent manner. Wild-type Tub4p is localized to the spindle pole body, and a Tub4p-green fluorescent protein fusion is able to associate with a preexisting spindle pole body, suggesting that there is dynamic exchange between cytoplasmic and spindle pole body forms of Tub4p. Perturbation of Tub4p function, either by conditional mutation or by depletion of the protein, results in spindle as well as spindle pole body defects, but does not eliminate the ability of microtubules to regrow from, or remain attached to, the spindle pole body. The spindle pole bodies in tub4 mutant cells duplicate but do not separate, resulting in a monopolar spindle. EM revealed that one spindle pole body of the duplicated pair appears to be defective for the nucleation of microtubules. These results offer insight into the role of gamma-tubulin in microtubule-organizing center function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.