ArticleThe Journal of cell biology1996
Coordinated regulation of platelet actin filament barbed ends by gelsolin and capping protein.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 144 citations in OpenAlex.
- A novel association between platelet filamin A and soluble N-ethylmaleimide sensitive factor attachment proteins regulates granule secretion.Research and practice in thrombosis and haemostasis · 2023Article
- A Ciliary View of the Immunological Synapse.Cells · 2019Review
- Article
- CPI motif interaction is necessary for capping protein function in cells.Nature communications · 2015Article
- Heterodimeric capping protein from Arabidopsis is a membrane-associated, actin-binding protein.Plant physiology · 2014Article
- Platelets and cancer: a casual or causal relationship: revisited.Cancer metastasis reviews · 2014Review
- Wiskott-Aldrich syndrome protein (WASp) controls the delivery of platelet transforming growth factor-β1.The Journal of biological chemistry · 2013Article
- Prognostic value of CAPZA1 overexpression in gastric cancer.International journal of oncology · 2013Article
- Rho GTPases in platelet function.Journal of thrombosis and haemostasis : JTH · 2013Review
- Capping protein modulates the dynamic behavior of actin filaments in response to phosphatidic acid in Arabidopsis.The Plant cell · 2012Article
- Defective erythroid maturation in gelsolin mutant mice.Haematologica · 2012Article
- Immunological responses and actin dynamics in macrophages are controlled by N-cofilin but are independent from ADF.PloS one · 2012Article
- Measurements of spatiotemporal changes in G-actin concentration reveal its effect on stimulus-induced actin assembly and lamellipodium extension.The Journal of cell biology · 2011Article
- Article
- Direct dynamin-actin interactions regulate the actin cytoskeleton.The EMBO journal · 2010Article
- CapZ dynamics are altered by endothelin-1 and phenylephrine via PIP2- and PKC-dependent mechanisms.American journal of physiology. Cell physiology · 2009Article
- New insights into mechanism and regulation of actin capping protein.International review of cell and molecular biology · 2008Review
- Rac1 and Rac2 differentially regulate actin free barbed end formation downstream of the fMLP receptor.The Journal of cell biology · 2007Article
- A major role for Scar/WAVE-1 downstream of GPVI in platelets.Journal of thrombosis and haemostasis : JTH · 2007Article
- Structure/function analysis of the interaction of phosphatidylinositol 4,5-bisphosphate with actin-capping protein: implications for how capping protein binds the actin filament.The Journal of biological chemistry · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Exposure of cryptic actin filament fast growing ends (barbed ends) initiates actin polymerization in stimulated human and mouse platelets. Gelsolin amplifies platelet actin assembly by severing F-actin and increasing the number of barbed ends. Actin filaments in stimulated platelets from transgenic gelsolin-null mice elongate their actin without severing. F-actin barbed end capping activity persists in human platelet extracts, depleted of gelsolin, and the heterodimeric capping protein (CP) accounts for this residual activity. 35% of the approximately 5 microM CP is associated with the insoluble actin cytoskeleton of the resting platelet. Since resting platelets have an F-actin barbed end concentration of approximately 0.5 microM, sufficient CP is bound to cap these ends. CP is released from OG-permeabilized platelets by treatment with phosphatidylinositol 4,5-bisphosphate or through activation of the thrombin receptor. However, the fraction of CP bound to the actin cytoskeleton of thrombin-stimulated mouse and human platelets increases rapidly to approximately 60% within 30 s. In resting platelets from transgenic mice lacking gelsolin, which have 33% more F-actin than gelsolin-positive cells, there is a corresponding increase in the amount of CP associated with the resting cytoskeleton but no change with stimulation. These findings demonstrate an interaction between the two major F-actin barbed end capping proteins of the platelet: gelsolin-dependent severing produces barbed ends that are capped by CP. Phosphatidylinositol 4,5-bisphosphate release of gelsolin and CP from platelet cytoskeleton provides a mechanism for mediating barbed end exposure. After actin assembly, CP reassociates with the new actin cytoskeleton.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.