ArticleThe Journal of cell biology1996
Evidence that phospholipase D mediates ADP ribosylation factor-dependent formation of Golgi coated vesicles.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
96 citing papers in PubMed, 380 citations in OpenAlex.
- Golgi retention of KIT in gastrointestinal stromal tumour cells is phospholipase D activity-dependent.Scientific reports · 2025Article
- Arfs on the Golgi: four conductors, one orchestra.Frontiers in molecular biosciences · 2025Review
- Membrane Curvature, Trans-Membrane Area Asymmetry, Budding, Fission and Organelle Geometry.International journal of molecular sciences · 2020Review
- Mammalian phospholipase D: Function, and therapeutics.Progress in lipid research · 2020Review
- Giantin Is Required for Post-Alcohol Recovery of Golgi in Liver Cells.Biomolecules · 2018Article
- Study of Ethanol-Induced Golgi Disorganization Reveals the Potential Mechanism of Alcohol-Impaired N-Glycosylation.Alcoholism, clinical and experimental research · 2016Article
- Selective protection of an ARF1-GTP signaling axis by a bacterial scaffold induces bidirectional trafficking arrest.Cell reports · 2014Article
- Regulation of the Golgi complex by phospholipid remodeling enzymes.Biochimica et biophysica acta · 2012Review
- Phospholipase D: enzymology, functionality, and chemical modulation.Chemical reviews · 2011Review
- Janus-faced enzymes yeast Tgl3p and Tgl5p catalyze lipase and acyltransferase reactions.Molecular biology of the cell · 2010Article
- Mechanisms of COPI vesicle formation.FEBS letters · 2009Review
- Phospholipase D in the Golgi apparatus.Biochimica et biophysica acta · 2009Review
- Rhabdomere biogenesis in Drosophila photoreceptors is acutely sensitive to phosphatidic acid levels.The Journal of cell biology · 2009Article
- Design of isoform-selective phospholipase D inhibitors that modulate cancer cell invasiveness.Nature chemical biology · 2009Article
- Early stages of Golgi vesicle and tubule formation require diacylglycerol.Molecular biology of the cell · 2009Article
- A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance.Nature cell biology · 2008Article
- Membrane stress is coupled to a rapid translational control of gene expression in chlorpromazine-treated cells.Current genetics · 2007Article
- Overexpression of Arabidopsis AGD7 causes relocation of Golgi-localized proteins to the endoplasmic reticulum and inhibits protein trafficking in plant cells.Plant physiology · 2007Article
- Phospholipase D signaling: orchestration by PIP2 and small GTPases.Naunyn-Schmiedeberg's archives of pharmacology · 2007Review
- Role of lipids and actin in the formation of clathrin-coated pits.Experimental cell research · 2006Article
36 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Formation of coatomer-coated vesicles from Golgi-enriched membranes requires the activation of a small GTP-binding protein, ADP ribosylation factor (ARF). ARF is also an efficacious activator of phospholipase D (PLD), an activity that is relatively abundant on Golgi-enriched membranes. It has been proposed that ARF, which is recruited onto membranes from cytosolic pools, acts directly to promote coatomer binding and is in a 3:1 stoichiometry with coatomer on coated vesicles. We present evidence that cytosolic ARF is not necessary for initiating coat assembly on Golgi membranes from cell lines with high constitutive PLD activity. Conditions are also described under which ARF is at most a minor component relative to coatomer in coated vesicles from all cell lines tested, including Chinese hamster ovary cells. Formation of coated vesicles was sensitive to ethanol at concentrations that inhibit the production of phosphatidic acid (PA) by PLD. When PA was produced in Golgi membranes by an exogenous bacterial PLD, rather than with ARF and endogenous PLD, coatomer bound to Golgi membranes. Purified coatomer also bound selectively to artificial lipid vesicles that contained PA and phosphatidylinositol (4,5)-bisphosphate (PIP2). We propose that activation of PLD and the subsequent production of PA are key early events for the formation of coatomer-coated vesicles.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.