ArticleGut1996
Leucocyte typing, cytokine expression, and epithelial turnover in the ileal pouch in patients with ulcerative colitis and familial adenomatous polyposis.
Article in Gut, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 47 citations in OpenAlex.
- Acute and chronic pouchitis--pathogenesis, diagnosis and treatment.Nature reviews. Gastroenterology & hepatology · 2012Review
- Surgical prophylaxis of pouchitis in ulcerative colitis.Digestive diseases and sciences · 2011Review
- Diagnosis and treatment of pouchitis.Gastroenterology & hepatology · 2008Article
- Pouchitis.World journal of gastroenterology · 2007Review
- Increased epithelial cell proliferation in the ileal pouch mucosa of patients with familial adenomatous polyposis.Virchows Archiv : an international journal of pathology · 2007Article
- Pouchitis: a spectrum of diseases.Current gastroenterology reports · 2005Review
- CXC and CC chemokine expression in inflamed and noninflamed pelvic ileal pouch tissue.International journal of colorectal disease · 2004Article
- Activation of signal-transducer and activator of transcription 1 (STAT1) in pouchitis.Clinical and experimental immunology · 2001Article
- Experimental colitis induced by trinitrobenzenesulfonic acid: an ultrastructural and histochemical study.Digestive diseases and sciences · 1999Article
- Article
- Selective binding of nucleotide probes by eosinophilic cationic protein during in situ hybridisation.The Histochemical journalArticle
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND/
aimsConventional histopathology, leucocyte typing, cytokine mRNA expression, and crypt cell turnover were compared in ileal pouch biopsy specimens from patients with ulcerative colitis (UC) and familial adenomatous polyposis (FAP).
methodsBiopsy specimens were taken from 17 patients with UC and seven with FAP at a median interval of 19 months (range 2-120) after ileostomy closure. All contained both epithelium and lamina propria. Cryostat sections were stained for lymphocyte subtypes (CD3, CD4, CD8), macrophages (CD68), common leucocyte antigen (CD45), and Ki-67, using a three stage immunoperoxidase reaction. Cytokine mRNA expression for interleukins 2 and 6, tumour necrosis factor alpha, and interferon gamma was studied using an in situ hybridisation technique.
resultsLymphocyte subtype and macrophage populations in epithelium and lamina propria were similar in UC and FAP. The labelling index (Ki-67) was significantly increased in biopsy specimens from patients with UC (UC median = 43.3 (interquartile range (IQR) 38.9-48.2) v FAP 34.9 (29.9-35.2), p < 0.05). There was little or no epithelial mRNA expression for any cytokine in any of the specimens. Lamina propria mRNA expression for interleukin 2 was significantly increased in UC (UC median (IQR) 10.7 (5.4-14.2) cells per unit area v FAP 2.8 (1.5-6.6) p < 0.05) but not for tumour necrosis factor alpha, interleukin 6, and interferon gamma.
conclusionsWhile static morphological assessment (leucocyte type, conventional histopathological examination) was similar, tests of cell function (mRNA expression and labelling index) were different in ileal pouches in patients with UC compared with FAP. The study also showed that mRNA expression occurred almost entirely in the lamina propria.
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