ArticleClinical and diagnostic laboratory immunology1996
Enzyme-linked immunosorbent assay with partially purified cytosoluble 28-kilodalton protein for serological differentiation between Brucella melitensis-infected and B. melitensis Rev.1-vaccinated sheep.
Article in Clinical and diagnostic laboratory immunology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Development and evaluation of the recombinant BP26 protein-based C-ELISA for human brucellosis diagnosis.Frontiers in microbiology · 2024Article
- Simultaneous Immunization with Omp25 and L7/L12 Provides Protection against Brucellosis in Mice.Pathogens (Basel, Switzerland) · 2020Article
- Recombinant Omp2b antigen-based ELISA is an efficient tool for specific serodiagnosis of animal brucellosis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): infection withEFSA journal. European Food Safety Authority · 2017Article
- Serological diagnostic potential of recombinant outer membrane proteins (rOMPs) from Brucella melitensis in mouse model using indirect enzyme-linked immunosorbent assay.BMC veterinary research · 2015Article
- Effect of Varying the Energy Density of Protein-adequate Diets on Nutrient Metabolism, Clinical Chemistry, Immune Response and Growth of Muzaffarnagari Lambs.Asian-Australasian journal of animal sciences · 2013Article
- Article
- Characterization of periplasmic protein BP26 epitopes of Brucella melitensis reacting with murine monoclonal and sheep antibodies.PloS one · 2012Article
- Systems biology approach predicts antibody signature associated with Brucella melitensis infection in humans.Journal of proteome research · 2011Article
- Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens.PLoS neglected tropical diseases · 2010Article
- P26-based serodiagnosis for Bartonella spp. infection in cats.Comparative medicine · 2008Article
- Cloning, characterization, and expression of Bartonella henselae p26.Clinical and vaccine immunology : CVI · 2006Article
- Selection of protective epitopes for Brucella melitensis by DNA vaccination.Infection and immunity · 2005Article
- Epitope mapping of the Brucella melitensis BP26 immunogenic protein: usefulness for diagnosis of sheep brucellosis.Clinical and diagnostic laboratory immunology · 2003Article
- Cloning, nucleotide sequence, and expression of the Brucella melitensis sucB gene coding for an immunogenic dihydrolipoamide succinyltransferase homologous protein.Infection and immunity · 2001Article
- Use of recombinant BP26 protein in serological diagnosis of Brucella melitensis infection in sheep.Clinical and diagnostic laboratory immunology · 2001Article
- Performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis.Clinical and diagnostic laboratory immunology · 1999Article
- Evaluation of lipopolysaccharides and polysaccharides of different epitopic structures in the indirect enzyme-linked immunosorbent assay for diagnosis of brucellosis in small ruminants and cattle.Clinical and diagnostic laboratory immunology · 1998Article
- Antibody and delayed-type hypersensitivity responses to Ochrobactrum anthropi cytosolic and outer membrane antigens in infections by smooth and rough Brucella spp.Clinical and diagnostic laboratory immunology · 1997Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The problem of differentiating sheep infected with Brucella melitensis from those vaccinated or exposed to cross-reaching organisms has not been resolved by conventional serological tests or through the use of the smooth lipopolysaccharide in primary binding assays. We therefore analyzed sera from ewes experimentally infected with B. melitensis H38, from ewes naturally infected with B. melitensis, and from B. melitensis Rev.1-vaccinated ewes by enzyme-linked immunosorbent assay with three antigenic fractions: O polysaccharide, a cytosoluble protein extract (CPE) from the rough strain B. melitensis B115, and a partially purified cytosoluble protein of 28 kDa (CP28) from the CPE. Immunoglobulin G anti-O polysaccharide and anti-CPE responses were detected in all groups of animals tested (Rev.1 vaccinated and B. melitensis infected). However, false-positive reactions with CPE occurred with sera from Brucella-free ewes. The use of partially purified CP28 abolished these false-positive reactions. Furthermore, no immunoglobulin G antibodies against CP28 were detected in sera from vaccinated ewes, whereas 80% (8 of 10) of ewes experimentally infected with B. melitensis H38 and 89% (25 of 28) of naturally infected ewes showed various degrees of anti-CP28 reactivity (absorbance values of between 0.5 and 2.5). The results obtained with CP28 showed the potential usefulness of this antigen to permit the detection of B. melitensis-infected ewes and their differentiation from B. melitensis Rev.1-vaccinated ones.
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