ArticlePlant molecular biology1996
A novel kinesin-like protein with a calmodulin-binding domain.
Article in Plant molecular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 61 citations in OpenAlex.
- Calmodulin-like protein AtCML3 mediates dimerization of peroxisomal processing protease AtDEG15 and contributes to normal peroxisome metabolism.Plant molecular biology · 2013Article
- Recent advances in calcium/calmodulin-mediated signaling with an emphasis on plant-microbe interactions.Plant physiology · 2013Review
- Article
- Efficient RNA polyuridylation by noncanonical poly(A) polymerases.Molecular and cellular biology · 2007Article
- Microtubule-associated proteins in higher plants.Journal of plant research · 2007Review
- Signalling pathways in pollen germination and tube growth.Protoplasma · 2006Review
- Origin and evolution of Kinesin-like calmodulin-binding protein.Plant physiology · 2005Article
- KIC, a novel Ca2+ binding protein with one EF-hand motif, interacts with a microtubule motor protein and regulates trichome morphogenesis.The Plant cell · 2004Article
- Developmental and cell-specific expression of ZWICHEL is regulated by the intron and exon sequences of its gene.Plant molecular biology · 2004Article
- Molecular cloning and functional analysis of mouse C-terminal kinesin motor KifC3.Molecular and cellular biology · 2001Article
- Kinesins in the Arabidopsis genome: a comparative analysis among eukaryotes.BMC genomics · 2001Article
- Identification and characterization of a novel microtubule-based motor associated with membranous organelles in tobacco pollen tubes.The Plant cell · 2000Article
- Cell cycle regulation of the microtubular cytoskeleton.Plant molecular biology · 2000Review
- The kinesin-like calmodulin binding protein is differentially involved in cell division.The Plant cell · 2000Article
- Characterization of microtubule binding domains in the Arabidopsis kinesin-like calmodulin binding protein.The Plant cell · 1998Article
- Neocentromere-mediated chromosome movement in maize.The Journal of cell biology · 1997Article
- In vitro motility of AtKCBP, a calmodulin-binding kinesin protein of Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calcium regulates diverse developmental processes in plants through the action of calmodulin. A cDNA expression library from developing anthers of tobacco was screened with 35S-labeled calmodulin to isolate cDNAs encoding calmodulin-binding proteins. Among several clones isolated, a kinesin-like gene (TCK1) that encodes a calmodulin-binding kinesin-like protein was obtained. The TCK1 cDNA encodes a protein with 1265 amino acid residues. Its structural features are very similar to those of known kinesin heavy chains and kinesin-like proteins from plants and animals, with one distinct exception. Unlike other known kinesin-like proteins, TCK1 contains a calmodulin-binding domain which distinguishes it from all other known kinesin genes. Escherichia coli-expressed TCK1 binds calmodulin in a Ca(2+)-dependent manner. In addition to the presence of a calmodulin-binding domain at the carboxyl terminal, it also has a leucine zipper motif in the stalk region. The amino acid sequence at the carboxyl terminal of TCK1 has striking homology with the mechanochemical motor domain of kinesins. The motor domain has ATPase activity that is stimulated by microtubules. Southern blot analysis revealed that TCK1 is coded by a single gene. Expression studies indicated that TCK1 is expressed in all of the tissues tested. Its expression is highest in the stigma and anther, especially during the early stages of anther development. Our results suggest that Ca2+/calmodulin may play an important role in the function of this microtubule-associated motor protein and may be involved in the regulation of microtubule-based intracellular transport.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.