ArticlePlant molecular biology1996
Isolation of a full-length cDNA encoding calreticulin from a PCR library of in vitro zygotes of maize.
Article in Plant molecular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 85 citations in OpenAlex.
- Functional Analysis and Marker Development ofFrontiers in plant science · 2017Article
- Late progamic phase and fertilization affect calreticulin expression in the Hyacinthus orientalis female gametophyte.Plant cell reports · 2015Article
- Article
- Overexpression of a Triticum aestivum Calreticulin gene (TaCRT1) Improves Salinity Tolerance in Tobacco.PloS one · 2015Article
- Article
- Occurrence of calreticulin during the exchange of nucleohistones into protamine-type proteins in Chara vulgaris spermiogenesis.Protoplasma · 2013Article
- Calreticulin expression and localization in plant cells during pollen-pistil interactions.Planta · 2009Article
- Calreticulin mRNA and protein are localized to protein bodies in storage maize callus cells.Plant cell reports · 2008Article
- EST generation and analyses towards identifying female gametophyte-specific genes in Zea mays L.Planta · 2006Article
- Article
- The fertilization-induced DNA replication factor MCM6 of maize shuttles between cytoplasm and nucleus, and is essential for plant growth and development.Plant physiology · 2006Article
- TRANSPARENT LEAF AREA1 encodes a secreted proteolipid required for anther maturation, morphogenesis, and differentiation during leaf development in maize.The Plant cell · 2005Article
- Some reflections on double fertilization, from its discovery to the present.The New phytologist · 2003Review
- Expression of the high capacity calcium-binding domain of calreticulin increases bioavailable calcium stores in plants.Transgenic research · 2002Article
- Organ specificity of a vacuolar Ca2+-binding protein RVCaB in radish and its expression under Ca2+-deficient conditions.Plant molecular biology · 2001Article
- Transient dissociation of polyribosomes and concurrent recruitment of calreticulin and calmodulin transcripts in gravistimulated maize pulvini.Plant physiology · 2001Article
- Purification, properties, and molecular cloning of a novel Ca(2+)-binding protein in radish vacuoles.Plant physiology · 2000Article
- Calreticulin: one protein, one gene, many functions.The Biochemical journal · 1999Review
- The endoplasmic reticulum of plant cells and its role in protein maturation and biogenesis of oil bodies.Plant molecular biology · 1998Review
- BiP and calreticulin form an abundant complex that is independent of endoplasmic reticulum stressThe Plant cell · 1998Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A full-size cDNA clone (1614 bp) encoding calreticulin was isolated from a PCR-based cDNA library of maize in vitro zygotes. Calreticulin is a major Ca2+ storage protein located mainly in the lumen of the endoplasmic reticulum but also in the nucleus and/or cytoplasm of some cells. A differential screening between cDNA libraries originating from 104 in vitro zygotes (18 h after in vitro fertilization) and 128 unfertilized egg cells was performed to isolate newly expressed genes or genes expressed more abundantly after fertilization. The expression of the isolated cDNA clone is enhanced after fertilization and strongly correlated to cell division. Sequence comparison to a shorter maize calreticulin cDNA isolated from a PCR based cDNA library construction from a few plant cells [12]. It is further shown that calreticulins in maize are probably transcribed from a small gene family differentially expressed in abundance in diverse tissues. The deduced amino acid sequence encodes an acidic protein (pI 4.17) of 48 kDa sharing 77-92% and 50-54% homology to other plant and animal calreticulins, respectively. The described calreticulin gene represents to our knowledge the first cDNA clone isolated from a RT/PCR cDNA library originating from only a few plant cells and is the first gene isolated from zygotes of higher plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.