ArticlePlant molecular biology1996
Expression of the Volvox gene encoding nitrate reductase: mutation-dependent activation of cryptic splice sites and intron-enhanced gene expression from a cDNA.
Article in Plant molecular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Septin-mediated coupling of protein import and division during chloroplast evolution.bioRxiv : the preprint server for biology · 2026Article
- Spatiotemporal distribution of the glycoprotein pherophorin II reveals stochastic geometry of the growing ECM ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Functional analysis of regA paralog rlsD in Volvox carteri.The Plant journal : for cell and molecular biology · 2024Article
- Cell Type-Specific Promoters ofGenes · 2023Article
- Cell Type-Specific Pherophorins ofCells · 2022Article
- Molecular and cellular dynamics of early embryonic cell divisions in Volvox carteri.The Plant cell · 2022Article
- Volvox and volvocine green algae.EvoDevo · 2020Review
- Designing a new generation of expression toolkits for engineering of green microalgae; robust production of human interleukin-2.BioImpacts : BI · 2020Article
- Two-component cyclase opsins of green algae are ATP-dependent and light-inhibited guanylyl cyclases.BMC biology · 2018Article
- Identification of Chlamydomonas reinhardtii endogenous genic flanking sequences for improved transgene expression.The Plant journal : for cell and molecular biology · 2017Article
- Evolution of sexes from an ancestral mating-type specification pathway.PLoS biology · 2014Article
- Manipulating RuBisCO accumulation in the green alga, Chlamydomonas reinhardtii.Plant molecular biology · 2011Article
- Controlled enlargement of the glycoprotein vesicle surrounding a volvox embryo requires the InvB nucleotide-sugar transporter and is required for normal morphogenesis.The Plant cell · 2009Article
- Article
- A gender-specific retinoblastoma-related protein in Volvox carteri implies a role for the retinoblastoma protein family in sexual development.The Plant cell · 2008Article
- Maintenance DNA methyltransferase (Met1) and silencing of CpG-methylated foreign DNA in Volvox carteri.Plant molecular biology · 2007Article
- The role of GlsA in the evolution of asymmetric cell division in the green alga Volvox carteri.Development genes and evolution · 2003Article
- Two enhancers and one silencer located in the introns of regA control somatic cell differentiation in Volvox carteri.Genes & development · 2001Article
- A link between DNA methylation and epigenetic silencing in transgenic Volvox carteri.Nucleic acids research · 2001Article
- Gene replacement by homologous recombination in the multicellular green alga Volvox carteri.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
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3 authors.
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Abstract
Use of the nitrate reductase encoding gene (nitA) as selection marker has facilitated the successful nuclear transformation of Volvox carteri. The Volvox nitA gene contains 10 introns. A stable nitA mutation in the Volvox recipient strain 153-81 resides in a G-to-A transition of the first nucleotide in the 5' splice site of nitA intron 2. This mutation resulted in at least three non-functional splice variants, namely: (1) intron 2 was not spliced at all; (2) a cryptic 5' splice site 60 nt upstream or (3) a cryptic 5' splice site 16 nt downstream of the mutation were activated and used for splicing. When we used nitA cDNA (pVcNR13) for transformation of V. carteri 153-81, a low efficiency of about 5 x 10(-5) transformants per reproductive cell was observed. Re-integration of either intron 1 (pVcNR15) or introns 9 and 10 (pVcNR16) in the transforming cDNA increased transformation rates to 5 x 10(-4). In parallel, pVcNR15-transformed Volvox exhibited growth rates that were 100-fold increased over the pVcNR13-transformed alga. This intron-enhancement of nitA gene expression appears to be associated with post-transcriptional processing and 'channelling' of the message. These data suggest an important role of splicing for gene expression in V. carteri.
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