ArticlePlant molecular biology1996
Calcium-dependent protein kinase gene expression in response to physical and chemical stimuli in mungbean (Vigna radiata).
Article in Plant molecular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Mechanical stimulation in plants: molecular insights, morphological adaptations, and agricultural applications in monocots.BMC biology · 2025Review
- Insights on Calcium-Dependent Protein Kinases (CPKs) Signaling for Abiotic Stress Tolerance in Plants.International journal of molecular sciences · 2019Review
- Poplar stem transcriptome is massively remodelled in response to single or repeated mechanical stimuli.BMC genomics · 2017Article
- Comprehensive Analysis of the CDPK-SnRK Superfamily Genes in Chinese Cabbage and Its Evolutionary Implications in Plants.Frontiers in plant science · 2017Article
- Rice small C2-domain proteins are phosphorylated by calcium-dependent protein kinase.Molecules and cells · 2013Article
- Functional characterisation of OsCPK21, a calcium-dependent protein kinase that confers salt tolerance in rice.Plant molecular biology · 2011Article
- AtCPK6, a functionally redundant and positive regulator involved in salt/drought stress tolerance in Arabidopsis.Planta · 2010Article
- Expressional analysis and role of calcium regulated kinases in abiotic stress signaling.Current genomics · 2010Article
- Evolutionary and functional study of the CDPK gene family in wheat (Triticum aestivum L.).Plant molecular biology · 2008Article
- A chemical-genetic approach to elucidate protein kinase function in planta.Plant molecular biology · 2007Article
- Expression analysis of calcium-dependent protein kinase gene family during reproductive development and abiotic stress conditions in rice (Oryza sativa L. ssp. indica).Molecular genetics and genomics : MGG · 2007Article
- A wound-responsive and phospholipid-regulated maize calcium-dependent protein kinase.Plant physiology · 2005Article
- Article
- Abscisic acid promoted changes in the protein profiles of rice seedling by proteome analysis.Molecular biology reports · 2004Article
- Autophosphorylation and subcellular localization dynamics of a salt- and water deficit-induced calcium-dependent protein kinase from ice plant.Plant physiology · 2004Article
- Calcium in plants.Annals of botany · 2003Review
- Regulation of CDPK isoforms during tuber development.Plant molecular biology · 2003Article
- The Arabidopsis CDPK-SnRK superfamily of protein kinases.Plant physiology · 2003Article
- Transcriptional activation of a maize calcium-dependent protein kinase gene in response to fungal elicitors and infection.Plant molecular biology · 2001Article
- Enhanced expression of a calcium-dependent protein kinase from the moss Funaria hygrometrica under nutritional starvation.Journal of biosciences · 2000Article
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4 authors.
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Abstract
Protein kinases are important in eukaryotic signal transduction pathways. In this study we designed degenerate oligonucleotides corresponding to two conserved regions of protein kinases and using the polymerase chain reaction (PCR) have amplified a 141 bp fragment of DNA from mungbeans (Vigna radiata Rwilcz cv. Berken). Sequence analysis of the PCR products indicates that they encode several putative protein kinases with respect to their identity with other known plant protein kinases. Using one of the six fragments (CPK3-8), we isolated a 2022 bp cDNA (VrCDPK-1) from a Vigna radiata lambda gt11 library. VrCDPK-1 has a 96 bp 5'-untranslated region and a 465 bp 3'-untranslated region and an open reading frame of 1461 bp. VrCDPK-1 contains all of the conserved regions commonly found in calcium dependent protein kinases (CDPK). VrCDPK-1 shares 24 to 89% sequence identity with previously reported sequences for plant CDPKs at the protein level. Southern analysis revealed the presence of several copies of the CDPK gene. VrCDPK-1 expression was stimulated when mungbean cuttings were treated with CaCl2, while treatment with MgCl2 had no effect. We are reporting for the first time a CDPK gene in mungbean which is inducible by mechanical strain. Cuttings treated with indole-3-acetic acid (IAA) or subjected to salt stress showed an increase in VrCDPK-1 expression. There was a dramatic stimulation in VrCDPK-1 expression 6 h after cuttings were treated with cycloheximide.
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