ArticleThe Journal of biological chemistry1996
Identification and cloning of centaurin-alpha. A novel phosphatidylinositol 3,4,5-trisphosphate-binding protein from rat brain.
Article in The Journal of biological chemistry, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 143 citations in OpenAlex.
- Article
- ArfGAP with Dual Pleckstrin Homology Domains 2 Promotes Hypertrophy of Cultured Neonatal Cardiomyocytes.International journal of molecular sciences · 2025Article
- Article
- Cancer stem cells and their niche in the progression of squamous cell carcinoma.Cancer science · 2020Review
- ADAP1 promotes invasive squamous cell carcinoma progression and predicts patient survival.Life science alliance · 2019Article
- ADAP1 limits neonatal cardiomyocyte hypertrophy by reducing integrin cell surface expression.Scientific reports · 2018Article
- ADAP2 Is an Interferon Stimulated Gene That Restricts RNA Virus Entry.PLoS pathogens · 2015Article
- Centaurin-α1-Ras-Elk-1 signaling at mitochondria mediates β-amyloid-induced synaptic dysfunction.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Phosphoinositide 3-kinase enhancer (PIKE) in the brain: is it simply a phosphoinositide 3-kinase/Akt enhancer?Reviews in the neurosciences · 2012Review
- Article
- Phosphorylation-independent dual-site binding of the FHA domain of KIF13 mediates phosphoinositide transport via centaurin alpha1.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Inositol trisphosphate 3-kinases: focus on immune and neuronal signaling.Cellular and molecular life sciences : CMLS · 2010Review
- Inositol trisphosphate 3-kinase B (InsP3KB) as a physiological modulator of myelopoiesis.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Inositol 1,3,4,5-tetrakisphosphate negatively regulates phosphatidylinositol-3,4,5- trisphosphate signaling in neutrophils.Immunity · 2007Article
- The neuronal Arf GAP centaurin alpha1 modulates dendritic differentiation.Journal of cell science · 2007Article
- The centaurin gamma-1 GTPase-like domain functions as an NTPase.The Biochemical journal · 2007Article
- Transport of PIP3 by GAKIN, a kinesin-3 family protein, regulates neuronal cell polarity.The Journal of cell biology · 2006Article
- Regulation of CDC42 GTPase by proline-rich tyrosine kinase 2 interacting with PSGAP, a novel pleckstrin homology and Src homology 3 domain containing rhoGAP protein.The Journal of cell biology · 2001Article
- ACAPs are arf6 GTPase-activating proteins that function in the cell periphery.The Journal of cell biology · 2000Article
- Signal-dependent membrane targeting by pleckstrin homology (PH) domains.The Biochemical journal · 2000Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Using an affinity resin and photoaffinity label based on phospholipid analogs of inositol 1,3,4,5-tetrakisphosphate (InsP4), we have isolated, characterized, and cloned a 46-kDa protein from rat brain, which we have named centaurin-alpha. Binding specificity was determined using displacement of 1-O-[3H](3-[4-benzoyldihydrocinnamidyl]propyl)-InsP4 photoaffinity labeling. Centaurin-alpha displayed highest affinity for phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) (IC50 = 120 nM), whereas InsP4, PtdInsP2, and InsP3 bound with 5-, 12-, and >50-fold lower affinity, respectively. Screening a rat brain cDNA library with a polymerase chain reaction product, generated using partial amino acid sequence from tryptic peptides, yielded a full-length clone. The 2,450-base pair cDNA contained an open reading frame (ORF) encoding a novel protein of 419 amino acids. Northern analysis revealed a 2.5-kilobase transcript that is highly expressed in brain. The deduced sequence contains a novel putative zinc finger motif, 10 ankyrin-like repeats, and shows homology to recently identified yeast and mammalian Arf GTPase-activating proteins. Given the specificity of binding and enrichment in brain, centaurin-alpha is a candidate PtdInsP3 receptor that may link the activation of phosphoinositide 3-kinase to downstream responses in the brain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.