ArticleProceedings of the National Academy of Sciences of the United States of America1996
An ATP-dependent As(III)-glutathione transport system in membrane vesicles of Leishmania tarentolae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 145 citations in OpenAlex.
- Insights into drug resistance inADMET & DMPK · 2026Review
- Validating Immunomodulatory Responses of r-Pathogens (Basel, Switzerland) · 2022Article
- Global distribution of treatment resistance gene markers for leishmaniasis.Journal of clinical laboratory analysis · 2022Review
- Glutathione-coordinated metal complexes as substrates for cellular transporters.Metallomics : integrated biometal science · 2021Review
- MRPA-independent mechanisms of antimony resistance in Leishmania infantum.International journal for parasitology. Drugs and drug resistance · 2020Article
- Drug resistance in eukaryotic microorganisms.Nature microbiology · 2016Review
- Species-specific antimonial sensitivity in Leishmania is driven by post-transcriptional regulation of AQP1.PLoS neglected tropical diseases · 2015Article
- The overexpression of genes of thiol metabolism contribute to drug resistance in clinical isolates of visceral leishmaniasis (kala azar) in India.Parasites & vectors · 2014Article
- Flavone-resistant Leishmania donovani overexpresses LdMRP2 transporter in the parasite and activates host MRP2 on macrophages to circumvent the flavone-mediated cell death.The Journal of biological chemistry · 2014Article
- Novel Arsenic Nanoparticles Are More Effective and Less Toxic than As (III) to Inhibit Extracellular and Intracellular Proliferation of Leishmania donovani.Journal of parasitology research · 2014Article
- A new ABC half-transporter in Leishmania major is involved in resistance to antimony.Antimicrobial agents and chemotherapy · 2013Article
- Role of efflux pumps and intracellular thiols in natural antimony resistant isolates of Leishmania donovani.PloS one · 2013Article
- Drug resistance in natural isolates of Leishmania donovani s.l. promastigotes is dependent of Pgp170 expression.PloS one · 2013Article
- Molecular mechanisms of drug resistance in natural Leishmania populations vary with genetic background.PLoS neglected tropical diseases · 2012Article
- Visceral leishmaniasis and arsenic: an ancient poison contributing to antimonial treatment failure in the Indian subcontinent?PLoS neglected tropical diseases · 2011Article
- Use of antimony in the treatment of leishmaniasis: current status and future directions.Molecular biology international · 2011Article
- Antimony resistance in leishmania, focusing on experimental research.Journal of tropical medicine · 2011Article
- Intracellular localization of the ABCC proteins of Leishmania and their role in resistance to antimonials.Antimicrobial agents and chemotherapy · 2009Article
- Gene expression levels in normal human lymphoblasts with variable sensitivities to arsenite: identification of GGT1 and NFKBIE expression levels as possible biomarkers of susceptibility.Toxicology and applied pharmacology · 2008Article
- Organization of H locus conserved repeats in Leishmania (Viannia) braziliensis correlates with lack of gene amplification and drug resistance.Parasitology research · 2007Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
Membrane preparations enriched in plasma membrane vesicles prepared from promastigotes of Leishmania tarentolae were shown to accumulate thiolate derivatives of 73As(III). Free arsenite was transported at a low rate, but rapid accumulation was observed after reaction with reduced glutathione (GSH) conditions that favor the formation of As(GS)3. Accumulation required ATP but not electrochemical energy, indicating that As(GS)3 is transported by an ATP-coupled pump. Pentostam, a Sb(V)-containing drug that is one of the first-line therapeutic agents for treatment of leishmaniasis, inhibited uptake after reaction with GSH. Vesicles prepared from a strain in which both copies of the pgpA genes were disrupted accumulated As(GS)3 at wild-type levels, demonstrating that the PgpA protein is not the As(GS)3 pump. These results have important implications for the mechanism of drug resistance in the trypanosomatidae, suggesting that a plasma membrane As(GS)3 pump catalyzes active extrusion of metal thiolates, including the Pentostam-glutathione conjugate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.