ArticleProceedings of the National Academy of Sciences of the United States of America1996
Inhibition of G-protein betagamma-subunit functions by phosducin-like protein.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 64 citations in OpenAlex.
- Structural Basis for the 14-3-3 Protein-Dependent Inhibition of Phosducin Function.Biophysical journal · 2017Article
- Identification of PDCL3 as a novel chaperone protein involved in the generation of functional VEGF receptor 2.The Journal of biological chemistry · 2013Article
- Functional Subunits of Eukaryotic Chaperonin CCT/TRiC in Protein Folding.Journal of amino acids · 2011Article
- Structure of the thioredoxin-fold domain of human phosducin-like protein 2.Acta crystallographica. Section F, Structural biology and crystallization communications · 2009Article
- Function of phosducin-like proteins in G protein signaling and chaperone-assisted protein folding.Cellular signalling · 2007Review
- Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeletal protein function and cell cycle progression.Molecular biology of the cell · 2007Article
- The phosducin-like protein PhLP1 is essential for G{beta}{gamma} dimer formation in Dictyostelium discoideum.Molecular and cellular biology · 2005Article
- Phosducin-like proteins in Dictyostelium discoideum: implications for the phosducin family of proteins.The EMBO journal · 2003Article
- Regulatory interaction of phosducin-like protein with the cytosolic chaperonin complex.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Modulation of CRX transactivation activity by phosducin isoforms.Molecular and cellular biology · 2000Article
- Structural features of heterotrimeric G-protein-coupled receptors and their modulatory proteins.Molecular neurobiology · 1999Review
- The amyloid precursor protein interacts with Go heterotrimeric protein within a cell compartment specialized in signal transduction.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- A small region in phosducin inhibits G-protein betagamma-subunit function.The EMBO journal · 1997Article
- Phosducin is a ubiquitous G-protein regulator.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
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2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Phosducin is a cytosolic protein predominantly expressed in the retina and the pineal gland that can interact with the betagamma subunits of guanine nucleotide binding proteins (G proteins) and thereby may regulate transmembrane signaling. A cDNA encoding a phosducin-like protein (PhLP) has recently been isolated from rat brain [Miles, M. F., Barhite, S., Sganga, M. & Elliott, M. (1993) Proc. Natl. Acad. Sci. USA 90, 10831-10835. Here we report the expression of PhLP in Escherichia coli and its purification. Recombinant purified PUP inhibited multiple effects of G-protein betagamma subunits. First, it inhibited the betagamma-subunit-dependent ADP-ribosylation of purified alpha(o) by pertussis toxin. Second, it inhibited the GTPase activity of purified G(o). The IC50 value of PhLP in the latter assay was 89 nM, whereas phosducin caused half-maximal inhibition at 17 nM. And finally, PhLP antagonized the enhancement of rhodopsin phosphorylation by purified betagamma subunits. The N terminus of PhLP shows no similarity to the much longer N terminus of phosducin, the region shown to be critical for phosducin-betagamma-subunit interactions. Therefore, PhLP appears to bind to G-protein betagamma subunits by an as yet unknown mode of interaction and may represent an endogenous regulator of G-protein function.
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