ArticleThe Journal of cell biology1996
The Saccharomyces cerevisiae actin-related protein Arp2 is involved in the actin cytoskeleton.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 127 citations in OpenAlex.
- Two ligands of Arp2/3 complex, yeast coronin and GMF, interact and synergize in pruning branched actin networks.The Journal of biological chemistry · 2025Article
- Fusarium oxysporum f. sp.mBio · 2023Article
- Structure of the nucleation-promoting factor SPIN90 bound to the actin filament nucleator Arp2/3 complex.The EMBO journal · 2018Article
- Identification of an ATP-controlled allosteric switch that controls actin filament nucleation by Arp2/3 complex.Nature communications · 2016Article
- Rac1 signaling in the establishment of the fucoid algal body plan.Frontiers in plant science · 2014Article
- Identification of prefoldin amplification (1q23.3-q24.1) in bladder cancer using comparative genomic hybridization (CGH) arrays of urinary DNA.Journal of translational medicine · 2013Article
- Insertions within the actin core of actin-related protein 3 (Arp3) modulate branching nucleation by Arp2/3 complex.The Journal of biological chemistry · 2013Article
- The p40/ARPC1 subunit of Arp2/3 complex performs multiple essential roles in WASp-regulated actin nucleation.The Journal of biological chemistry · 2010Article
- Forward genetics in Candida albicans that reveals the Arp2/3 complex is required for hyphal formation, but not endocytosis.Molecular microbiology · 2010Article
- The F-BAR protein Syp1 negatively regulates WASp-Arp2/3 complex activity during endocytic patch formation.Current biology : CB · 2009Article
- Polarized growth in the absence of F-actin in Saccharomyces cerevisiae exiting quiescence.PloS one · 2008Article
- Translation of ASH1 mRNA is repressed by Puf6p-Fun12p/eIF5B interaction and released by CK2 phosphorylation.Genes & development · 2008Article
- A novel plasmid-based microarray screen identifies suppressors of rrp6Delta in Saccharomyces cerevisiae.Molecular and cellular biology · 2007Article
- Puf3p, a Pumilio family RNA binding protein, localizes to mitochondria and regulates mitochondrial biogenesis and motility in budding yeast.The Journal of cell biology · 2007Article
- The yeast actin cytoskeleton: from cellular function to biochemical mechanism.Microbiology and molecular biology reviews : MMBR · 2006Review
- ARP2 and ARP3 are localized to sites of actin filament nucleation in tobacco BY-2 cells.Protoplasma · 2006Article
- The BAR domain proteins: molding membranes in fission, fusion, and phagy.Microbiology and molecular biology reviews : MMBR · 2006Review
- A role for Jsn1p in recruiting the Arp2/3 complex to mitochondria in budding yeast.Molecular biology of the cell · 2005Article
- The WASP/Las17p-interacting protein Bzz1p functions with Myo5p in an early stage of endocytosis.Protoplasma · 2005Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arp2p is an essential yeast actin-related protein. Disruption of the corresponding ARP2 gene leads to a terminal phenotype characterized by the presence of a single large bud. Thus, Arp2p may be important for a late stage of the cell cycle (Schwob, E., and R.P. Martin, 1992. Nature (Lond.). 355:179-182). We have localized Arp2p by indirect immunofluorescence. Specific peptide antibodies revealed punctate staining under the plasma membrane, which partially colocalizes with actin. Temperature-sensitive arp2 mutations were created by PCR mutagenesis and selected by an ade2/SUP11 sectoring screen. One temperature-sensitive mutant that was characterized, arp2-H330L, was osmosensitive and had an altered actin cytoskeleton at a nonpermissive temperature, suggesting a role of Arp2p in the actin cytoskeleton. Random budding patterns were observed in both haploid and diploid arp2-H330L mutant cells. Endocytosis, as judged by Lucifer yellow uptake, was severely reduced in the mutant, at all temperatures. In addition, genetic interaction was observed between temperature-sensitive alleles arp2-H330L and cdc10-1. CDC10 is a gene encoding a neck filament-associated protein that is necessary for polarized growth and cytokinesis. Overall, the immunolocalization, mutant phenotypes, and genetic interaction suggest that the Arp2 protein is an essential component of the actin cytoskeleton that is involved in membrane growth and polarity, as well as in endocytosis.
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