ArticleInfection and immunity1996
Cloning of a Brucella melitensis group 3 antigen gene encoding Omp28, a protein recognized by the humoral immune response during human brucellosis.
Article in Infection and immunity, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 72 citations in OpenAlex.
- A subunit vaccine based onActa biochimica et biophysica Sinica · 2024Article
- Brucellosis detection and the role ofVeterinary world · 2023Review
- A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis.BMC veterinary research · 2021Article
- Binding of Brucella protein, Bp26, to select extracellular matrix molecules.BMC molecular and cell biology · 2019Article
- Diagnostic potential ofCentral-European journal of immunology · 2018Article
- Evaluation of recombinant porin (rOmp2a) protein as a potential antigen candidate for serodiagnosis of Human Brucellosis.BMC infectious diseases · 2017Article
- Expression, purification and immunochemical characterization of recombinant OMP28 protein of Brucella species.Open veterinary journal · 2016Article
- Serological diagnostic potential of recombinant outer membrane proteins (rOMPs) from Brucella melitensis in mouse model using indirect enzyme-linked immunosorbent assay.BMC veterinary research · 2015Article
- Limitations of the BP26 protein-based indirect enzyme-linked immunosorbent assay for diagnosis of Brucellosis.Clinical and vaccine immunology : CVI · 2013Article
- 16S rRNA and omp31 gene based molecular characterization of field strains of B. melitensis from aborted foetus of goats in India.TheScientificWorldJournal · 2013Article
- Protective effects of recombinant Brucella abortus Omp28 against infection with a virulent strain of Brucella abortus 544 in mice.Journal of veterinary science · 2012Article
- Article
- Systems biology approach predicts antibody signature associated with Brucella melitensis infection in humans.Journal of proteome research · 2011Article
- Evaluation of the recombinant 10-kilodalton immunodominant region of the BP26 protein of Brucella abortus for specific diagnosis of bovine brucellosis.Clinical and vaccine immunology : CVI · 2011Article
- Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens.PLoS neglected tropical diseases · 2010Article
- Protection of mice against Brucella abortus 544 challenge by vaccination with recombinant OMP28 adjuvanted with CpG oligonucleotides.Veterinary research communications · 2010Article
- P26-based serodiagnosis for Bartonella spp. infection in cats.Comparative medicine · 2008Article
- VirB12 is a serological marker of Brucella infection in experimental and natural hosts.Clinical and vaccine immunology : CVI · 2008Article
- Cloning, characterization, and expression of Bartonella henselae p26.Clinical and vaccine immunology : CVI · 2006Article
- Protection against brucellosis in goats, five years after vaccination with reduced-dose Brucella melitensis Rev 1 vaccine.Tropical animal health and production · 2004Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brucella group 3 antigens (Ags) are outer membrane proteins (OMPs) with a molecular mass ranging from 25 to 30 kDa. The OMPs are of interest partially because of their potential use as vaccine and diagnostic reagents. We used human convalescent antibody (Ab) to clone a gene that encoded a 28-kDa protein from a lambdagt11 library of Brucella melitensis 16M genomic DNA. DNA sequence analysis revealed a single open reading frame that would encode a protein of 26,552 Da. The 28-kDa protein had a primary amino acid sequence that was 43% similar to a previously described Brucella abortus group 3 Ag, Omp25 (P. de Wergifosse, P. Lintermans, J. N. Limet, and A. Cloeckaert, J. Bacteriol. 177:1911-1914, 1995). The similarity to a known group 3 OMP, immunoreactivity with Ab prepared against B. abortus group Ags, immunolabeling of whole cells, and Southern hybridization led to our conclusion that the B. melitensis 28-kDa protein was a group 3 protein distinct from B. abortus Omp25. We designated the B. melitensis protein Omp28. Human convalescent sera from patients infected with B. abortus and Brucella suis as well as rabbit antisera prepared against killed B. abortus whole cells recognized B. melitensis Omp28 on Western blots (immunoblots). Furthermore, mice and goats infected with smooth strains of B. melitensis produced Abs against Omp28. Our results may begin to explain the variability in molecular weight seen in Brucella group Ags and point toward their possible use in vaccination against infection as well as diagnosis of the disease.
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