ArticleThe Biochemical journal1996
Affinity purification of 5-methylthioribose kinase and 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Klebsiella pneumoniae [corrected].
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Avian PathogenicVeterinary sciences · 2022Article
- Pathway Driven Target Selection inFrontiers in cellular and infection microbiology · 2022Article
- Gut Microbiota Composition and Its Metabolites in Different Stages of Chronic Kidney Disease.Journal of clinical medicine · 2021Article
- Genome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genes.Scientific reports · 2020Article
- Methylthioadenosine/S-adenosylhomocysteine nucleosidase, a critical enzyme for bacterial metabolism.Molecular microbiology · 2011Review
- Assessment of methylthioadenosine/S-adenosylhomocysteine nucleosidases of Borrelia burgdorferi as targets for novel antimicrobials using a novel high-throughput method.The Journal of antimicrobial chemotherapy · 2009Article
- Characterization of chromosomal regions conserved in Yersinia pseudotuberculosis and lost by Yersinia pestis.Infection and immunity · 2008Article
- Structure of Arabidopsis thaliana 5-methylthioribose kinase reveals a more occluded active site than its bacterial homolog.BMC structural biology · 2007Article
- Studies on the regulation of ornithine decarboxylase in yeast: effect of deletion in the MEU1 gene.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Functional analysis of methylthioribose kinase genes in plants.Plant physiology · 2004Article
- Branched-chain amino acid aminotransferase and methionine formation in Mycobacterium tuberculosis.BMC microbiology · 2004Article
- pfs-dependent regulation of autoinducer 2 production in Salmonella enterica serovar Typhimurium.Journal of bacteriology · 2002Article
- Prediction of gene function in methylthioadenosine recycling from regulatory signals.Journal of bacteriology · 2002Article
- MtnK, methylthioribose kinase, is a starvation-induced protein in Bacillus subtilis.BMC microbiology · 2001Article
- Tyrosine aminotransferase catalyzes the final step of methionine recycling in Klebsiella pneumoniae.Journal of bacteriology · 1999Article
- Metabolism of sulfur amino acids in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 1997Review
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Two enzymes in the methionine salvage pathway, 5-methylthioribose kinase (MTR kinase) and 5'-methylthioadenosine/ S-adenosylhomocysteine nucleosidase (MTA/SAH nucleosidase) were purified from Klebsiella pneumoniae. Chromatography using a novel 5'-(p-aminophenyl)thioadenosine/5-(p-aminophenyl)thioribose affinity matrix allowed the binding and selective elution of each of the enzymes in pure form. The molecular mass, substrate kinetics and N-terminal amino acid sequences were characterized for each of the enzymes. Purified MTR kinase exhibits an apparent molecular mass of 46-50 kDa by SDS/PAGE and S200HR chromatography, and has a Km for MTR of 12.2 microM. Homogeneous MTA/SAH nucleosidase displays a molecular mass of 26.5 kDa by SDS/PAGE, and a Km for MTA of 8.7 microM. Comparisons of the N-terminal sequences obtained for each of the enzymes with protein-sequence databases failed to reveal any significant sequence similarities to known proteins. However, the amino acid sequence obtained for the nucleosidase did share a high degree of sequence similarity with the putative translation product of an open reading frame in Escherichia coli, thus providing a tentative identification of this gene as encoding an MTA/SAH nucleosidase.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.