ArticleThe Biochemical journal1996
Cloning and biochemical characterization of the cyclophilin homologues from the free-living nematode Caenorhabditis elegans.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 62 citations in OpenAlex.
- Structural Basis for Cyclosporin Isoform-Specific Inhibition of Cyclophilins fromACS infectious diseases · 2023Article
- Characterization of Cyclophilin from ThaumarchaeotaACS omega · 2022Article
- Immunoproteomic Analysis ofPathogens (Basel, Switzerland) · 2021Article
- Plant Cyclophilins: Multifaceted Proteins With Versatile Roles.Frontiers in plant science · 2020Review
- Enzyme activity and structural features of three single-domain phloem cyclophilins from Brassica napus.Scientific reports · 2019Article
- The immunophilin repertoire of Plasmodiophora brassicae and functional analysis of PbCYP3 cyclophilin.Molecular genetics and genomics : MGG · 2018Article
- Soybean cyclophilin GmCYP1 interacts with an isoflavonoid regulator GmMYB176.Scientific reports · 2017Article
- Article
- Cyclophilins: proteins in search of function.Plant signaling & behavior · 2013Review
- Streamlined, automated protocols for the production of milligram quantities of untagged recombinant human cyclophilin-A (hCypA) and untagged human proliferating cell nuclear antigen (hPCNA) using AKTAxpress.Protein expression and purification · 2010Article
- Heterologous expression of a salinity and developmentally regulated rice cyclophilin gene (OsCyp2) in E. coli and S. cerevisiae confers tolerance towards multiple abiotic stresses.Molecular biotechnology · 2009Article
- Brugia malayi excreted/secreted proteins at the host/parasite interface: stage- and gender-specific proteomic profiling.PLoS neglected tropical diseases · 2009Article
- Identification and comparative analysis of the peptidyl-prolyl cis/trans isomerase repertoires of H. sapiens, D. melanogaster, C. elegans, S. cerevisiae and Sz. pombe.Comparative and functional genomics · 2005Article
- Cyclophilin nomenclature problems, or, 'a visit from the sequence police'.Human genomics · 2004Review
- Two protein-protein interaction sites on the spliceosome-associated human cyclophilin CypH.Nucleic acids research · 2003Article
- Calcineurin, a calcium/calmodulin-dependent protein phosphatase, is involved in movement, fertility, egg laying, and growth in Caenorhabditis elegans.Molecular biology of the cell · 2002Article
- Prolyl 4-hydroxylase is an essential procollagen-modifying enzyme required for exoskeleton formation and the maintenance of body shape in the nematode Caenorhabditis elegans.Molecular and cellular biology · 2000Article
- A conserved domain of the arabidopsis GNOM protein mediates subunit interaction and cyclophilin 5 binding.The Plant cell · 2000Article
- Identification of abundantly expressed novel and conserved genes from the infective larval stage of Toxocara canis by an expressed sequence tag strategy.Infection and immunity · 1999Article
- Leishmania major parasites express cyclophilin isoforms with an unusual interaction with calcineurin.The Biochemical journal · 1998Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyclosporin A (CsA) is the most widely used immunosuppressive agent, whose properties are exerted via an interaction with cyclophilin, resulting in down-regulation of signal-transduction events in the T-cell. Cyclophilin is identical with peptidylprolyl cis-trans isomerase (PPI; EC 5.2.1.8), an enzyme which catalyses the isomerization between the two proline conformations in proteins, thereby acting as a catalyst in protein-folding events. Several reports indicate that CsA has potent anti-parasitic activity, effective against both protozoan and helminth species. In order to understand the various biological roles that cyclophilins play we have initiated a study of these proteins in the genetically tractable nematode Caenorhabditis elegans. Here we describe the cloning and characterization of 11 cyclophilin genes (cyp-1 to -11) derived from this nematode; this is currently the greatest number of isoforms described in a single species. Southern blotting and physical mapping indicated that these genes are dispersed throughout the nematode genome. A high degree of conservation exists between several isoforms, which also share characteristics with the ubiquitous isoforms previously described. The remaining isoforms are divergent, having altered CsA-binding domains and additional non-cyclophilin domains, which may impart compartmental specificity. Ten of these isoforms have been expressed in Escherichia coli, and the resultant fusion proteins have been examined biochemically for PPI activity, which they all possess. Isomerase activity is highest in the conserved and lowest in divergent isoforms, perhaps indicating a more specific substrate for the latter. Analysis of the C. elegans cyp genes will provide answers as to the roles played by cyclophilins in protein folding and signal transduction.
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