ArticleProceedings of the National Academy of Sciences of the United States of America1996
Brush border myosin-I truncated in the motor domain impairs the distribution and the function of endocytic compartments in an hepatoma cell line.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- The role of motor proteins in photoreceptor protein transport and visual function.Ophthalmic genetics · 2022Article
- Deciphering the role of UBA-like domains in intraflagellar distribution and functions of myosin XXI in Leishmania.PloS one · 2020Article
- Calmodulin dissociation regulates Myo5 recruitment and function at endocytic sites.The EMBO journal · 2010Article
- Multiple myosins are required to coordinate actin assembly with coat compression during compensatory endocytosis.Molecular biology of the cell · 2007Article
- Myosin-1a is critical for normal brush border structure and composition.Molecular biology of the cell · 2005Article
- A two-photon FRAP analysis of the cytoskeleton dynamics in the microvilli of intestinal cells.Biophysical journal · 2005Article
- Functional characterization of myosin I tail regions in Candida albicans.Eukaryotic cell · 2004Article
- A role for myosin-1A in the localization of a brush border disaccharidase.The Journal of cell biology · 2004Article
- The role of mVps18p in clustering, fusion, and intracellular localization of late endocytic organelles.Molecular biology of the cell · 2003Article
- Cofilin, but not profilin, is required for myosin-I-induced actin polymerization and the endocytic uptake in yeast.Molecular biology of the cell · 2002Article
- Nonpolarized cells selectively sort apical proteins from cell surface to a novel compartment, but lack apical retention mechanisms.Molecular biology of the cell · 2002Article
- Myosin I is required for hypha formation in Candida albicans.Eukaryotic cell · 2002Article
- Evidence of a role for nonmuscle myosin II in herpes simplex virus type 1 egress.Journal of virology · 2002Article
- MYO1A (brush border myosin I) dynamics in the brush border of LLC-PK1-CL4 cells.Biophysical journal · 2002Article
- Actin filaments and myosin I alpha cooperate with microtubules for the movement of lysosomes.Molecular biology of the cell · 2001Article
- Fission yeast myosin-I, Myo1p, stimulates actin assembly by Arp2/3 complex and shares functions with WASp.The Journal of cell biology · 2000Article
- Association of myosin I alpha with endosomes and lysosomes in mammalian cells.Molecular biology of the cell · 1999Article
- Unconventional myosins at the crossroad of signal transduction and cytoskeleton remodeling.Protoplasma · 1999Article
- Disruption of the talin gene compromises focal adhesion assembly in undifferentiated but not differentiated embryonic stem cells.The Journal of cell biology · 1998Article
- Fluid-phase markers in the basolateral endocytic pathway accumulate in response to the actin assembly-promoting drug Jasplakinolide.Molecular biology of the cell · 1998Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myosins I, a ubiquitous monomeric class of myosins that exhibits actin-based motor properties, are associated with plasma and/or vesicular membranes and have been suggested as players for trafficking events between cell surface and intracellular membranous structures. To investigate the function of myosins 1, we have transfected a mouse hepatoma cell line (BWTG3) with cDNAs encoding the chicken brush border myosin-I (BBMI) and two variants truncated in the motor domain. One variant is deleted of the first 446 amino acids and thereby lacks the ATP binding site, whereas the other is deleted of the entire motor domain and lacks the ATP and actin binding sites. We have observed (i) that significant amounts of the truncated variants are recovered with membrane fractions after cell fractionation, (ii) that they codistribute with a compartment containing alpha2-macroglobulin internalized for 30 min as determined by fluorescent microscopy, (iii) that the production of BBMI-truncated variants impairs the distribution of the acidic compartment and ligands internalized for 30 min, and (iv) that the production of the truncated variant containing the actin binding site decreases the rate of alpha2-macroglobulin degradation whereas the production of the variant lacking the ATP binding site and the actin binding site increases the rate of a2-macroglobulin degradation. These observations indicate that the two truncated variants have a dominant negative effect on the distribution and the function of the endocytic compartments. We propose that an unidentified myosin-I might contribute to the distribution of endocytic compartments in a juxtanuclear position and/or to the regulation of the delivery of ligands to the degradative compartment in BWTG3 cells.
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