ArticleProceedings of the National Academy of Sciences of the United States of America1996
Identifying functional domains within terpene cyclases using a domain-swapping strategy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 112 citations in OpenAlex.
- Coevolutionary Information Captures Catalytic Functions and Reveals Divergent Roles of Terpene Synthase Interdomain Connections.Biochemistry · 2024Article
- Predictive Engineering of Class I Terpene Synthases Using Experimental and Computational Approaches.Chembiochem : a European journal of chemical biology · 2022Review
- Germacrene A-A Central Intermediate in Sesquiterpene Biosynthesis.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Review
- Structural and Chemical Biology of Terpenoid Cyclases.Chemical reviews · 2017Review
- Plant Glandular Trichomes: Natural Cell Factories of High Biotechnological Interest.Plant physiology · 2017Review
- Exploring the Influence of Domain Architecture on the Catalytic Function of Diterpene Synthases.Biochemistry · 2017Article
- The Enzymology of Organic Transformations: A Survey of Name Reactions in Biological Systems.Angewandte Chemie (International ed. in English) · 2017Review
- Molecular Diversity of Terpene Synthases in the Liverwort Marchantia polymorpha.The Plant cell · 2016Article
- Plant terpenes: defense responses, phylogenetic analysis, regulation and clinical applications.3 Biotech · 2015Article
- Reprogramming the chemodiversity of terpenoid cyclization by remolding the active site contour of epi-isozizaene synthase.Biochemistry · 2014Article
- S-carvone suppresses cellulase-induced capsidiol production in Nicotiana tabacum by interfering with protein isoprenylation.Plant physiology · 2014Article
- Terpenoid synthase structures: a so far incomplete view of complex catalysis.Natural product reports · 2012Review
- 3DSwap: curated knowledgebase of proteins involved in 3D domain swapping.Database : the journal of biological databases and curation · 2011Article
- Insights into Protein Sequence and Structure-Derived Features Mediating 3D Domain Swapping Mechanism using Support Vector Machine Based Approach.Bioinformatics and biology insights · 2010Article
- Article
- Diversity, regulation, and genetic manipulation of plant mono- and sesquiterpenoid biosynthesis.Cellular and molecular life sciences : CMLS · 2009Review
- Biochemical and genomic characterization of terpene synthases in Magnolia grandiflora.Plant physiology · 2008Article
- Elucidation of the biosynthetic origin of the anti-inflammatory pseudopterosins.Journal of industrial microbiology & biotechnology · 2006Review
- Identifying and manipulating structural determinates linking catalytic specificities in terpene synthases.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Stereochemistry and deuterium isotope effects associated with the cyclization-rearrangements catalyzed by tobacco epiaristolochene and hyoscyamus premnaspirodiene synthases, and the chimeric CH4 hybrid cyclase.Archives of biochemistry and biophysics · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyclic terpenes and terpenoids are found throughout nature. They comprise an especially important class of compounds from plants that mediate plant- environment interactions, and they serve as pharmaceutical agents with antimicrobial and anti-tumor activities. Molecular comparisons of several terpene cyclases, the key enzymes responsible for the multistep cyclization of C10, C15, and C20 allylic diphosphate substrates, have revealed a striking level of sequence similarity and conservation of exon position and size within the genes. Functional domains responsible for a terminal enzymatic step were identified by swapping regions approximating exons between a Nicotiana tabacum 5-epi-aristolochene synthase (TEAS) gene and a Hyoscyamus muticus vetispiradiene synthase (HVS) gene and by characterization of the resulting chimeric enzymes expressed in bacteria. While exon 4 of the TEAS gene conferred specificity for the predominant reaction products of the tobacco enzyme, exon 6 of the HVS gene conferred specificity for the predominant reaction products of the Hyoscyamus enzyme. Combining these two functional domains of the TEAS and HVS genes resulted in a novel enzyme capable of synthesizing reaction products reflective of both parent enzymes. The relative ratio of the TEAS and HVS reaction products was also influenced by the source of exon 5 present in the new chimeric enzymes. The association of catalytic activities with conserved but separate exonic domains suggests a general means for generating additional novel terpene cyclases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.