ArticleProceedings of the National Academy of Sciences of the United States of America1996
Efficient and specific ribozyme-mediated reduction of bovine alpha-lactalbumin expression in double transgenic mice.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 57 citations in OpenAlex.
- Ribozyme rescue of photoreceptor cells in P23H transgenic rats: long-term survival and late-stage therapy.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Dose-response resistance to HIV-1/MuLV pseudotype virus ex vivo in a hairpin ribozyme transgenic mouse model.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Modification and repression of genes expressed in the mammary gland using gene targeting and other technologies.Journal of mammary gland biology and neoplasia · 1998Review
- Regulation of CAT protein by ribozyme and antisense mRNA in transgenic mice.Transgenic research · 1998Article
- Antiviral ribozymes. New jobs for ancient molecules.Molecular biotechnology · 1997Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transgenic mice carrying a bovine alpha-lactalbumin (alpha-lac) specific ribozyme gene under the transcriptional control of the mouse mammary tumor virus long terminal repeat were generated and cross-bred with animals that highly express a bovine alpha-lac transgene (0.4 mg of alpha-lac/ml(-1) of milk). The ribozyme contains the hammerhead catalytic domain, flanked by 12-nt sequences complementary to the 3' untranslated region of bovine alpha-lac transcript. High-level expression of the ribozyme gene was detected by Northern blot analysis in the mammary gland of 7-8 day lactating transgenic mice, from 3 of 12 lines analyzed. Heterozygous expression of the ribozyme resulted in a reduction in the levels of the target mRNA to 78, 58, and 50% of that observed in the nonribozyme transgenic littermate controls for three independent lines. The ribozyme-mediated reduction in the levels of the bovine protein paralleled that observed for the mRNA, and was positively correlated with the level of expression of the ribozyme. In nonribozyme expressing transgenic mice, the level of bovine alpha-lac mRNA and protein was not affected. The specificity of this activity is demonstrated by the absence of a reduction in the levels of the endogenous murine alpha-lac mRNA or protein. These results demonstrate the feasibility of ribozyme-mediated down-regulation of highly-expressed transcripts in transgenic animals.
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