ArticleProceedings of the National Academy of Sciences of the United States of America1996
Copy-choice recombination mediated by DNA polymerase III holoenzyme from Escherichia coli.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 64 citations in OpenAlex.
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- Guardians of the Genome: How the Single-Stranded DNA-Binding Proteins RPA and CST Facilitate Telomere Replication.Biomolecules · 2024Review
- Specificity Distorted: Chemical Induction of Biological Paracatalysis.Biochemistry · 2020Article
- Comparative genome analysis of 52 fish species suggests differential associations of repetitive elements with their living aquatic environments.BMC genomics · 2018Article
- Unprecedented large inverted repeats at the replication terminus of circular bacterial chromosomes suggest a novel mode of chromosome rescue.Scientific reports · 2017Article
- Targeting antisense mitochondrial ncRNAs inhibits murine melanoma tumor growth and metastasis through reduction in survival and invasion factors.Oncotarget · 2016Article
- Intrastrand triplex DNA repeats in bacteria: a source of genomic instability.Nucleic acids research · 2015Article
- Down-regulation of the antisense mitochondrial non-coding RNAs (ncRNAs) is a unique vulnerability of cancer cells and a potential target for cancer therapy.The Journal of biological chemistry · 2014Article
- Replication slippage of the thermophilic DNA polymerases B and D from the Euryarchaeota Pyrococcus abyssi.Frontiers in microbiology · 2014Article
- Oligonucleotides stimulate genomic alterations of Legionella pneumophila.Molecular microbiology · 2011Article
- Small variable segments constitute a major type of diversity of bacterial genomes at the species level.Genome biology · 2010Article
- Strand displacement by DNA polymerase III occurs through a tau-psi-chi link to single-stranded DNA-binding protein coating the lagging strand template.The Journal of biological chemistry · 2009Article
- A comparative approach to elucidate chloroplast genome replication.BMC genomics · 2009Article
- Alternative mechanisms of immune receptor diversity.Current opinion in immunology · 2007Review
- Expression of a novel non-coding mitochondrial RNA in human proliferating cells.Nucleic acids research · 2007Article
- Article
- Local repeat sequence organization of an intergenic spacer in the chloroplast genome of Chlamydomonas reinhardtii leads to DNA expansion and sequence scrambling: a complex mode of "copy-choice replication"?Journal of biosciences · 2001Article
- Instability of repetitive DNA sequences: the role of replication in multiple mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Replication slippage involves DNA polymerase pausing and dissociation.The EMBO journal · 2001Article
- Characterization of the uup locus and its role in transposon excisions and tandem repeat deletions in Escherichia coli.Journal of bacteriology · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Formation of deletions by recombination between short direct repeats is thought to involve either a break-join or a copy-choice process. The key step of the latter is slippage of the replication machinery between the repeats. We report that the main replicase of Escherichia coli, DNA polymerase III holoenzyme, slips between two direct repeats of 27 bp that flank an inverted repeat of approximately equal 300bp. Slippage was detected in vitro, on a single-stranded DNA template, in a primer extension assay. It requires the presence of a short (8 bp) G+C-rich sequence at the base of a hairpin that can form by annealing of the inverted repeats. It is stimulated by (i) high salt concentration, which might stabilize the hairpin, and (ii) two proteins that ensure the processivity of the DNA polymerase III holoenzyme: the single-stranded DNA binding protein and the beta subunit of the polymerase. Slippage is rather efficient under optimal reaction conditions because it can take place on >50% of template molecules. This observation supports the copy-choice model for recombination between short direct repeats.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.