ArticleNucleic acids research1996
Functional interaction between TFIIB and the Rpb9 (Ssu73) subunit of RNA polymerase II in Saccharomyces cerevisiae.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 57 citations in OpenAlex.
- Important role in transcription start site selection for the RNA polymerase II-TFIIE-TFIIH interface in Saccharomyces cerevisiae.Genetics · 2026Article
- Genetic dissection of transcription start site selection by RNA polymerase II in Saccharomyces cerevisiae.Genetics · 2026Article
- RNA polymerase II-TFIIE-TFIIH interface functions in transcription start site selection inbioRxiv : the preprint server for biology · 2025Article
- Genetic dissection of transcription start site selection by RNA Polymerase II inbioRxiv : the preprint server for biology · 2025Article
- RNA Polymerase II Activity Control of Gene Expression and Involvement in Disease.Journal of molecular biology · 2025Review
- Article
- The RNA polymerase II subunit RPB-9 recruits the integrator complex to terminate Caenorhabditis elegans piRNA transcription.The EMBO journal · 2021Article
- Universal promoter scanning by Pol II during transcription initiation in Saccharomyces cerevisiae.Genome biology · 2020Article
- RNA Polymerase II Trigger Loop Mobility: INDIRECT EFFECTS OF Rpb9.The Journal of biological chemistry · 2016Article
- Relationships of RNA polymerase II genetic interactors to transcription start site usage defects and growth in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2014Article
- Basic mechanisms of RNA polymerase II activity and alteration of gene expression in Saccharomyces cerevisiae.Biochimica et biophysica acta · 2013Review
- Evidence that RNA polymerase II and not TFIIB is responsible for the difference in transcription initiation patterns between Saccharomyces cerevisiae and Schizosaccharomyces pombe.Nucleic acids research · 2012Article
- Multiplex assay for condition-dependent changes in protein-protein interactions.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Functional consequences of subunit diversity in RNA polymerases II and V.Cell reports · 2012Article
- Mechanism of start site selection by RNA polymerase II: interplay between TFIIB and Ssl2/XPB helicase subunit of TFIIH.The Journal of biological chemistry · 2012Article
- Dissection of Pol II trigger loop function and Pol II activity-dependent control of start site selection in vivo.PLoS genetics · 2012Article
- Hmo1 directs pre-initiation complex assembly to an appropriate site on its target gene promoters by masking a nucleosome-free region.Nucleic acids research · 2011Article
- Position of the general transcription factor TFIIF within the RNA polymerase II transcription preinitiation complex.The EMBO journal · 2010Article
- Functions of Saccharomyces cerevisiae TFIIF during transcription start site utilization.Molecular and cellular biology · 2008Article
- Properties of an intergenic terminator and start site switch that regulate IMD2 transcription in yeast.Molecular and cellular biology · 2008Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Recessive mutations in the SSU71, SSU72 and SSU73 genes of Saccharomyces cerevisiae were identified as either suppressors or enhancers of a TFIIB defect (sua7-1) that confers both a cold-sensitive growth phenotype and a downstream shift in transcription start site selection. The SSU71 (TFG1) gene encodes the largest subunit of TFIIF and SSU72 encodes a novel protein that is essential for cell viability. Here we report that SSU73 is identical to RPB9, the gene encoding the 14.2 kDa subunit of RNA polymerase II. The ssu73-1 suppressor compensates for both the growth defect and the downstream shift in start site selection associated with sua7-1. These effects are similar to those of the ssu71-1 suppressor and distinct from the ssu72-1 enhancer. The ssu73-1 allele was retrieved and sequenced, revealing a nonsense mutation at codon 107. Consequently, ssu73-1 encodes a truncated form of Rpb9 lacking the C-terminal 16 amino acids. This Rpb9 derivative retains at least partial function since the ssu73-1 mutant exhibits none of the growth defects associated with rpb9 null mutants. However, in a SUA7+ background, ssu73-1 confers the same upstream shift at ADH1 as an rpb9 null allele. This suggests that the C-terminus of Rpb9 functions in start site selection and demonstrates that the previously observed effects of rpb9 mutations on start site selection are not necessarily due to complete loss of function. These results establish a functional interaction between TFIIB and the Rpb9 subunit of RNA polymerase II and suggest that these two components of the preinitiation complex interact during transcription start site selection.
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