ArticleThe Journal of experimental medicine1996
Activation and homologous desensitization of human endothelial cells by CD40 ligand, tumor necrosis factor, and interleukin 1.
Article in The Journal of experimental medicine, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 115 citations in OpenAlex.
- Abnormal germinal center reactions in systemic lupus erythematosus demonstrated by blockade of CD154-CD40 interactions.The Journal of clinical investigation · 2003Trial
- The causal role of intestinal microbiome in development of pre-eclampsia.Functional & integrative genomics · 2023Article
- The human glomerular endothelial cells are potent pro-inflammatory contributors in an in vitro model of lupus nephritis.Scientific reports · 2019Article
- Downregulation of GATA6 in mTOR-inhibited human aortic endothelial cells: effects on TNF-α-induced VCAM-1 expression and monocytic cell adhesion.American journal of physiology. Heart and circulatory physiology · 2019Article
- TNF inhibits catecholamine production from induced sympathetic neuron-like cells in rheumatoid arthritis and osteoarthritis in vitro.Scientific reports · 2018Article
- Platelets and cancer angiogenesis nexus.Cancer metastasis reviews · 2017Review
- Group B streptococcal colonization and the risk of pre-eclampsia.Epidemiology and infection · 2013Article
- β3-Adrenergic receptor stimulation induces E-selectin-mediated adipose tissue inflammation.The Journal of biological chemistry · 2013Article
- JNK and NADPH oxidase involved in fluoride-induced oxidative stress in BV-2 microglia cells.Mediators of inflammation · 2013Article
- Ferritin as a novel reporter gene for photoacoustic molecular imaging.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2012Article
- Detection and monitoring of the multiple inflammatory responses by photoacoustic molecular imaging using selectively targeted gold nanorods.Biomedical optics express · 2011Article
- Identification of a ligand-induced transient refractory period in nuclear factor-kappaB signaling.The Journal of biological chemistry · 2008Article
- Synergistic induction of tissue factor by coagulation factor Xa and TNF: evidence for involvement of negative regulatory signaling cascades.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Endothelial cells present antigens in vivo.BMC immunology · 2004Article
- Upregulation of CD40 expression on endothelial cells infected with human cytomegalovirus.Journal of virology · 2002Article
- Endothelial activation: intracellular signaling pathways.Arthritis research · 2002Review
- Nuclear factor kappa B is involved in lipopolysaccharide-stimulated induction of interferon regulatory factor-1 and GAS/GAF DNA-binding in human umbilical vein endothelial cells.British journal of pharmacology · 2001Article
- Kinases: positive and negative regulators of apoptosis.Leukemia · 2000Review
- Article
- Differential regulation of CD4 lymphocyte recruitment between the upper and lower regions of the genital tract during Chlamydia trachomatis infection.Infection and immunity · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have reported previously that activation of human umbilical vein endothelial cells (HUVECs) through CD40, using a recombinant soluble form of trimerized CD40 ligand, leads to induction of E-selectin, vascular cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 (ICAM-1). Here, we compare the effects of CD40 ligand with those of tumor necrosis factor (TNF) and interleukin 1 (IL-1). All three ligands induce transient increases in E-selectin (peak 4 h) and VCAM-1 (peak 8-24 h), as well as sustained increases in ICAM-1 (plateau 24 h). Quantitatively, TNF is more potent than IL-1, which is much more potent than CD40 ligand. The same hierarchy is observed for transcriptional activation of an E-selectin promoter reporter gene construct in transiently transfected HUVECs. TNF and CD40 ligand each induced activation of the transcription factors NF-kappa B, IRF-1, and ATF-2/c-Jun, measured by electrophoretic mobility shift assays, but this response appeared quantitatively similar. All three agents transiently (peak 30 min) activated Jun NH2-terminal kinase (JNK), which has been implicated in transcription of E-selectin through its actions on ATF-2/c-Jun. Activation of JNK again showed a hierarchy of potency (TNF > IL-1 >> CD40 ligand), although the time course of induction was similar for all three agents. After 44 h of pretreatment, TNF, IL-1, and CD40 ligand each display homologous desensitization for reinduction of surface expression of E-selectin. A similar pattern of homologous desensitization for reactivation of JNK was observed. We conclude that TNF, IL-1, and CD40 ligand all activate similar responses in ECs, and that homologous desensitization of JNK may explain the inability of individual cytokines to reinduce E-selectin expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.