ArticleJournal of assisted reproduction and genetics1996
Reduction in signal overlap results in increased FISH efficiency: implications for preimplantation genetic diagnosis.
Article in Journal of assisted reproduction and genetics, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Pre-implantation genetic testing: Past, present, future.Reproductive medicine and biology · 2021Review
- Clinical application of oligo array-CGH for detecting balanced translocations in preimplantation genetic diagnosis.International journal of clinical and experimental pathology · 2017Article
- Pre-implantation genetic screening using fluorescence in situ hybridization in couples of Indian ethnicity: Is there a scope?Journal of human reproductive sciences · 2014Article
- Bioinformatics tools allow targeted selection of chromosome enumeration probes and aneuploidy detection.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2013Article
- False positive rate of an arrayCGH platform for single-cell preimplantation genetic screening and subsequent clinical application on day-3.Journal of assisted reproduction and genetics · 2013Article
- Somatic genomic variations in extra-embryonic tissues.Current genomics · 2010Article
- Higher degree of chromosome mosaicism in preimplantation embryos from carriers of robertsonian translocation t(13;14) in comparison with embryos from karyotypically normal IVF patients.Journal of assisted reproduction and genetics · 2003Article
- Germ-cell nondisjunction in testes biopsies of men with idiopathic infertility.American journal of human genetics · 1999Article
- Preimplantation genetic diagnosis of aneuploidy: were we looking at the wrong chromosomes?Journal of assisted reproduction and genetics · 1999Article
- Improving the fixation method for preimplantation genetic diagnosis by fluorescent in situ hybridization.Journal of assisted reproduction and genetics · 1998Article
- Spectral imaging in preconception/preimplantation genetic diagnosis of aneuploidy: multicolor, multichromosome screening of single cells.Journal of assisted reproduction and genetics · 1998Article
- Preimplantation genetic diagnosis.Journal of assisted reproduction and genetics · 1996Article
- Analysis of clinical outcomes and meiotic segregation modes following preimplantation genetic testing for structural rearrangements using aCGH/NGS in couples with balanced chromosome rearrangement.Reproductive medicine and biologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn the absence of mosaicism, one of the problems of preimplantation genetic diagnosis with FISH is the occurrence of false-negative hybridization results. It has been hypothesized that missing signals are produced by spatial overlap of signals. METHODS AND
resultsTo investigate the relation among cell density, signal overlap, and hybridization signal detection, 371 blastomeres and 4556 lymphocytes were fixed in different cellular concentrations and analyzed by FISH using probes for chromosomes X, Y, and 18, and their nuclear diameters and FISH results scored. The results showed that the lower the diameter of fixed nuclei, the higher the number of signal overlaps and missing signals. The minimum number of missing signals was obtained when lymphocyte and blastomere nuclei had 40 or more microns in diameter after fixation and FISH. Since blastomeres were fixed individually, results with blastomeres were invariably better than with lymphocytes.
Indexed as
Identifiers
8688588What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.