ArticleJournal of assisted reproduction and genetics1996
Detection of chromosomal abnormalities in human preimplantation embryos using FISH.
Article in Journal of assisted reproduction and genetics, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Randomized comparison of next-generation sequencing and array comparative genomic hybridization for preimplantation genetic screening: a pilot study.BMC medical genomics · 2015Trial
- Application of improved single blastomere fixation technique in preimplantation genetic diagnosis.Cytotechnology · 2020Article
- Selection of competent blastocysts for transfer by combining time-lapse monitoring and array CGH testing for patients undergoing preimplantation genetic screening: a prospective study with sibling oocytes.BMC medical genomics · 2014Article
- Incidence of chromosomal mosaicism in morphologically normal nonhuman primate preimplantation embryos.Fertility and sterility · 2010Article
- Effects of Timing on Cell Biopsy from Pre-compacted Morula Stage Bovine Embryos on Subsequent Embryonic Development.Journal of reproduction & infertility · 2010Article
- The relationship of pronuclear stage morphology and chromosome status at cleavage stage.Journal of assisted reproduction and genetics · 2003Article
- Incidence of chromosomal abnormalities from a morphologically normal cohort of embryos in poor-prognosis patients.Journal of assisted reproduction and genetics · 1998Article
- Preimplantation genetic diagnosis.Journal of assisted reproduction and genetics · 1996Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeMulticolour FISH has been used for the preimplantation diagnosis of sex for X-linked disorders and to examine the chromosome constitution of early human embryos. MATERIALS AND
methodsSingle blastomeres and whole embryos were spread using HCI and Tween 20. Multicolour FISH was performed using directly-labelled human DNA probes for chromosomes X, Y, and 1 in a two hour FISH procedure.
resultsFour groups of chromosome arrangements have been found in human preimplantation embryos (i) normal, all nuclei uniformly diploid, (ii) diploid mosaics, majority of the nuclei diploid, with a small number of nuclei aneuploid (iii) chromosomally abnormal, all nuclei uniformly chromosomally abnormal, e.g. XO, XXY, XXX and (iv) chaotic, all nuclei showing different chromosome complements.
conclusionsFor the preimplantation diagnosis of sex, an XX nucleus has always been representative of a female embryo. However, for the diagnosis of dominant disorders or chromosome abnormalities, two cells should be analysed to reduce the chance of misdiagnosis which may arise from chromosomal mosaicism. Implantation and further embryo development may be possible from mosaic or chromosomally abnormal embryos, but those showing chaotic chromosome arrangements would be unlikely to implant.
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